Thermo Scientific™

ProPac™ IMAC-10 HPLC Columns

Catalog number: 063710
Thermo Scientific™

ProPac™ IMAC-10 HPLC Columns

Catalog number: 063710
Resolve target proteins using a single column in a high-resolution gradient run with Thermo Scientific™ ProPac™ IMAC-10 LC Columns. These analytical and semi-preparative HPLC columns provides high-purity separations of metal-binding proteins and high loading capacity for protein purification applications. The use of small, nonporous resin particles results in narrow, high-efficiency peaks, with minimal band broadening due to dispersion. Use loading columns to charge the ProPac IMAC-10 LC columns but avoid contact of the metal solution with the system.
 
Catalog Number
063710
Unit Size
Each
Particle Size
10 μm
Diameter (Metric)
9 mm
Length (Metric)
50 mm
Column Format
Loading Column
Price (USD)
Full specifications
Column FormatLoading Column
Diameter (Metric)9 mm
Flow Rate0.1 to 10 mL/min
For Use WithProPac IMAC-10 Column
Length (Metric)50 mm
Max. Pressure3000 psi (207 bar)
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeLoading Column
Unit SizeEach
Catalog Number
Unit Size
Each
Particle Size
10 μm
Diameter (Metric)
2 mm
Length (Metric)
50 mm
Column Format
Analytical Column
Price (USD)
Full specifications
Column FormatAnalytical Column
Diameter (Metric)2 mm
Flow Rate1 mL/min
For Use WithUltiMate 3000 HPLC System
Length (Metric)50 mm
Max. Pressure3000 psi (207 bar)
Mobile Phase CompatibilitypH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeAnalytical Column
Unit SizeEach
Catalog Number
Unit Size
Each
Particle Size
10 μm
Diameter (Metric)
2 mm
Length (Metric)
250 mm
Column Format
Analytical Column
Price (USD)
Full specifications
Column FormatAnalytical Column
Diameter (Metric)2 mm
Flow Rate1 mL/min
For Use WithUltiMate 3000 HPLC System
Length (Metric)250 mm
Max. Pressure3000 psi (207 bar)
Mobile Phase CompatibilitypH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeAnalytical Column
Unit SizeEach
Catalog Number
Unit Size
Each
Particle Size
10 μm
Diameter (Metric)
4 mm
Length (Metric)
50 mm
Column Format
Analytical Column
Price (USD)
Full specifications
Column FormatAnalytical Column
Diameter (Metric)4 mm
Flow Rate1 mL/min
For Use WithUltiMate 3000 HPLC System
Length (Metric)50 mm
Max. Pressure3000 psi (207 bar)
Mobile Phase CompatibilitypH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeAnalytical Column
Unit SizeEach
Catalog Number
Unit Size
Each
Particle Size
10 μm
Diameter (Metric)
4 mm
Length (Metric)
250 mm
Column Format
Analytical Column
Price (USD)
Full specifications
Column FormatAnalytical Column
Diameter (Metric)4 mm
Flow Rate1 mL/min
For Use WithUltiMate 3000 HPLC System
Length (Metric)250 mm
Max. Pressure3000 psi (207 bar)
Mobile Phase CompatibilitypH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeAnalytical Column
Unit SizeEach
Catalog Number
Unit Size
Each
Particle Size
10 μm
Diameter (Metric)
9 mm
Length (Metric)
250 mm
Column Format
Analytical Column
Price (USD)
Full specifications
Column FormatAnalytical Column
Diameter (Metric)9 mm
Flow Rate1 mL/min
For Use WithUltiMate 3000 HPLC System
Length (Metric)250 mm
Max. Pressure3000 psi (207 bar)
Mobile Phase CompatibilitypH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeAnalytical Column
Unit SizeEach
Catalog Number
Unit Size
-
Particle Size
10 μm
Diameter (Metric)
22 mm
Length (Metric)
250 mm
Column Format
Loading Column
Price (USD)
Full specifications
Column FormatLoading Column
Diameter (Metric)22 mm
Flow Rate0.1 to 10 mL/min
For Use WithUltiMate 3000 HPLC System
Length (Metric)250 mm
Max. Pressure3000 psi (207 bar)
Mobile Phase CompatibilitypH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeAnalytical Column
Unit Size-
Catalog Number
Unit Size
Each
Particle Size
10 μm
Diameter (Metric)
9 mm
Length (Metric)
50 mm
Column Format
Analytical Column
Price (USD)
Full specifications
Column FormatAnalytical Column
Diameter (Metric)9 mm
Flow Rate1 mL/min
For Use WithUltiMate 3000 HPLC System
Length (Metric)50 mm
Max. Pressure3000 psi (207 bar)
Mobile Phase CompatibilitypH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeAnalytical Column
Unit SizeEach
Catalog Number
Unit Size
Each
Particle Size
10 μm
Diameter (Metric)
1 mm
Length (Metric)
50 mm
Column Format
Analytical Column
Price (USD)
Full specifications
Column FormatAnalytical Column
Diameter (Metric)1 mm
Flow Rate1 mL/min
For Use WithUltiMate 3000 HPLC System
Length (Metric)50 mm
Max. Pressure3000 psi (207 bar)
Mobile Phase CompatibilitypH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeAnalytical Column
Unit SizeEach
Catalog Number
Unit Size
Each
Particle Size
10 μm
Diameter (Metric)
4 mm
Length (Metric)
50 mm
Column Format
Loading Column
Price (USD)
Full specifications
Column FormatLoading Column
Diameter (Metric)4 mm
Flow Rate0.1 to 10 mL/min
For Use WithProPac IMAC-10 Column
Length (Metric)50 mm
Max. Pressure3000 psi (207 bar)
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeLoading Column
Unit SizeEach
Showing 10 of 11Show more
Catalog NumberSpecificationsUnit SizeParticle SizeDiameter (Metric)Length (Metric)Column FormatPrice (USD)
063710Full specifications
Each10 μm9 mm50 mmLoading ColumnRequest A Quote
Column FormatLoading Column
Diameter (Metric)9 mm
Flow Rate0.1 to 10 mL/min
For Use WithProPac IMAC-10 Column
Length (Metric)50 mm
Max. Pressure3000 psi (207 bar)
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeLoading Column
Unit SizeEach
063272Full specifications
Each 10 μm2 mm50 mmAnalytical ColumnContact Us ›
Column FormatAnalytical Column
Diameter (Metric)2 mm
Flow Rate1 mL/min
For Use WithUltiMate 3000 HPLC System
Length (Metric)50 mm
Max. Pressure3000 psi (207 bar)
Mobile Phase CompatibilitypH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeAnalytical Column
Unit SizeEach
063274Full specifications
Each10 μm2 mm250 mmAnalytical ColumnRequest A Quote
Column FormatAnalytical Column
Diameter (Metric)2 mm
Flow Rate1 mL/min
For Use WithUltiMate 3000 HPLC System
Length (Metric)250 mm
Max. Pressure3000 psi (207 bar)
Mobile Phase CompatibilitypH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeAnalytical Column
Unit SizeEach
063276Full specifications
Each 10 μm4 mm50 mmAnalytical ColumnContact Us ›
Column FormatAnalytical Column
Diameter (Metric)4 mm
Flow Rate1 mL/min
For Use WithUltiMate 3000 HPLC System
Length (Metric)50 mm
Max. Pressure3000 psi (207 bar)
Mobile Phase CompatibilitypH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeAnalytical Column
Unit SizeEach
063278Full specifications
Each 10 μm4 mm250 mmAnalytical ColumnContact Us ›
Column FormatAnalytical Column
Diameter (Metric)4 mm
Flow Rate1 mL/min
For Use WithUltiMate 3000 HPLC System
Length (Metric)250 mm
Max. Pressure3000 psi (207 bar)
Mobile Phase CompatibilitypH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeAnalytical Column
Unit SizeEach
063280Full specifications
Each10 μm9 mm250 mmAnalytical ColumnRequest A Quote
Column FormatAnalytical Column
Diameter (Metric)9 mm
Flow Rate1 mL/min
For Use WithUltiMate 3000 HPLC System
Length (Metric)250 mm
Max. Pressure3000 psi (207 bar)
Mobile Phase CompatibilitypH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeAnalytical Column
Unit SizeEach
063282Full specifications
-10 μm22 mm250 mmLoading ColumnRequest A Quote
Column FormatLoading Column
Diameter (Metric)22 mm
Flow Rate0.1 to 10 mL/min
For Use WithUltiMate 3000 HPLC System
Length (Metric)250 mm
Max. Pressure3000 psi (207 bar)
Mobile Phase CompatibilitypH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeAnalytical Column
Unit Size-
063615Full specifications
Each10 μm9 mm50 mmAnalytical ColumnRequest A Quote
Column FormatAnalytical Column
Diameter (Metric)9 mm
Flow Rate1 mL/min
For Use WithUltiMate 3000 HPLC System
Length (Metric)50 mm
Max. Pressure3000 psi (207 bar)
Mobile Phase CompatibilitypH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeAnalytical Column
Unit SizeEach
063617Full specifications
Each10 μm1 mm50 mmAnalytical ColumnRequest A Quote
Column FormatAnalytical Column
Diameter (Metric)1 mm
Flow Rate1 mL/min
For Use WithUltiMate 3000 HPLC System
Length (Metric)50 mm
Max. Pressure3000 psi (207 bar)
Mobile Phase CompatibilitypH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeAnalytical Column
Unit SizeEach
063667Full specifications
Each 10 μm4 mm50 mmLoading ColumnContact Us ›
Column FormatLoading Column
Diameter (Metric)4 mm
Flow Rate0.1 to 10 mL/min
For Use WithProPac IMAC-10 Column
Length (Metric)50 mm
Max. Pressure3000 psi (207 bar)
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeLoading Column
Unit SizeEach
Showing 10 of 11Show more
Tailored Specificity for IMAC Separation of Proteins
  • Retention control by imidazole concentration or pH gradient
  • Stable between pH 2 to 12, compatible with common protein purification reagents (nonionic detergents, reducing agents)
  • Effective under native or denaturing HPLC conditions
  • Wide range of metal-specific applications, including the separation of His-tagged protein aggregation variants, prion peptide analysis and separation of monoclonal antibodies
  • Shipped free from any metal and ready to be charged with the metal of your choice.
  • Reusable columns with metal tailored specificity
  • Resolve target proteins using a single column in a high resolution gradient run
  • High purity separations of metal-binding proteins
Includes: 10µm, nonporous, polymeric beads coated with a hydrophilic layer, then grafted with poly(IDA) chains.