Thermo Scientific™

ProPac™ IMAC-10 HPLC Columns

Name: ProPac™ IMAC-10 HPLC Columns
SKU: 063710

Catalog number: 063710

Related applications: Chromatography

Technical SupportCustomer Service

Thermo Scientific™

ProPac™ IMAC-10 HPLC Columns

Catalog number: 063710

Related applications: Chromatography

Technical SupportCustomer Service

Resolve target proteins using a single column in a high-resolution gradient run with Thermo Scientific™ ProPac™ IMAC-10 LC Columns. These analytical and semi-preparative HPLC columns provides high-purity separations of metal-binding proteins and high loading capacity for protein purification applications. The use of small, nonporous resin particles results in narrow, high-efficiency peaks, with minimal band broadening due to dispersion. Use loading columns to charge the ProPac IMAC-10 LC columns but avoid contact of the metal solution with the system.

Catalog Number

063710

Unit Size

Each

Particle Size

10 μm

Diameter (Metric)

9 mm

Length (Metric)

50 mm

Column Format

Loading Column

Price (USD)

Request A Quote

Full specifications

Column Format	Loading Column
Diameter (Metric)	9 mm
Flow Rate	0.1 to 10 mL/min
For Use With	ProPac IMAC-10 Column
Length (Metric)	50 mm
Max. Pressure	3000 psi (207 bar)
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Product Line	ProPac
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Loading Column
Unit Size	Each

Catalog Number

063272

Unit Size

Each

Particle Size

10 μm

Diameter (Metric)

2 mm

Length (Metric)

50 mm

Column Format

Analytical Column

Price (USD)

Full specifications

Column Format	Analytical Column
Diameter (Metric)	2 mm
Flow Rate	1 mL/min
For Use With	UltiMate 3000 HPLC System
Length (Metric)	50 mm
Max. Pressure	3000 psi (207 bar)
Mobile Phase Compatibility	pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Product Line	ProPac
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Analytical Column
Unit Size	Each

Catalog Number

063274

Unit Size

Each

Particle Size

10 μm

Diameter (Metric)

2 mm

Length (Metric)

250 mm

Column Format

Analytical Column

Price (USD)

Request A Quote

Full specifications

Column Format	Analytical Column
Diameter (Metric)	2 mm
Flow Rate	1 mL/min
For Use With	UltiMate 3000 HPLC System
Length (Metric)	250 mm
Max. Pressure	3000 psi (207 bar)
Mobile Phase Compatibility	pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Product Line	ProPac
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Analytical Column
Unit Size	Each

Catalog Number

063276

Unit Size

Each

Particle Size

10 μm

Diameter (Metric)

4 mm

Length (Metric)

50 mm

Column Format

Analytical Column

Price (USD)

Full specifications

Column Format	Analytical Column
Diameter (Metric)	4 mm
Flow Rate	1 mL/min
For Use With	UltiMate 3000 HPLC System
Length (Metric)	50 mm
Max. Pressure	3000 psi (207 bar)
Mobile Phase Compatibility	pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Product Line	ProPac
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Analytical Column
Unit Size	Each

Catalog Number

063278

Unit Size

Each

Particle Size

10 μm

Diameter (Metric)

4 mm

Length (Metric)

250 mm

Column Format

Analytical Column

Price (USD)

Full specifications

Column Format	Analytical Column
Diameter (Metric)	4 mm
Flow Rate	1 mL/min
For Use With	UltiMate 3000 HPLC System
Length (Metric)	250 mm
Max. Pressure	3000 psi (207 bar)
Mobile Phase Compatibility	pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Product Line	ProPac
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Analytical Column
Unit Size	Each

Catalog Number

063280

Unit Size

Each

Particle Size

10 μm

Diameter (Metric)

9 mm

Length (Metric)

250 mm

Column Format

Analytical Column

Price (USD)

Request A Quote

Full specifications

Column Format	Analytical Column
Diameter (Metric)	9 mm
Flow Rate	1 mL/min
For Use With	UltiMate 3000 HPLC System
Length (Metric)	250 mm
Max. Pressure	3000 psi (207 bar)
Mobile Phase Compatibility	pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Product Line	ProPac
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Analytical Column
Unit Size	Each

Catalog Number

063282

Unit Size

Particle Size

10 μm

Diameter (Metric)

22 mm

Length (Metric)

250 mm

Column Format

Loading Column

Price (USD)

Request A Quote

Full specifications

Column Format	Loading Column
Diameter (Metric)	22 mm
Flow Rate	0.1 to 10 mL/min
For Use With	UltiMate 3000 HPLC System
Length (Metric)	250 mm
Max. Pressure	3000 psi (207 bar)
Mobile Phase Compatibility	pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Analytical Column
Unit Size	-

Catalog Number

063615

Unit Size

Each

Particle Size

10 μm

Diameter (Metric)

9 mm

Length (Metric)

50 mm

Column Format

Analytical Column

Price (USD)

Request A Quote

Full specifications

Column Format	Analytical Column
Diameter (Metric)	9 mm
Flow Rate	1 mL/min
For Use With	UltiMate 3000 HPLC System
Length (Metric)	50 mm
Max. Pressure	3000 psi (207 bar)
Mobile Phase Compatibility	pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Product Line	ProPac
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Analytical Column
Unit Size	Each

Catalog Number

063617

Unit Size

Each

Particle Size

10 μm

Diameter (Metric)

1 mm

Length (Metric)

50 mm

Column Format

Analytical Column

Price (USD)

Request A Quote

Full specifications

Column Format	Analytical Column
Diameter (Metric)	1 mm
Flow Rate	1 mL/min
For Use With	UltiMate 3000 HPLC System
Length (Metric)	50 mm
Max. Pressure	3000 psi (207 bar)
Mobile Phase Compatibility	pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Product Line	ProPac
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Analytical Column
Unit Size	Each

Catalog Number

063667

Unit Size

Each

Particle Size

10 μm

Diameter (Metric)

4 mm

Length (Metric)

50 mm

Column Format

Loading Column

Price (USD)

Full specifications

Column Format	Loading Column
Diameter (Metric)	4 mm
Flow Rate	0.1 to 10 mL/min
For Use With	ProPac IMAC-10 Column
Length (Metric)	50 mm
Max. Pressure	3000 psi (207 bar)
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Product Line	ProPac
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Loading Column
Unit Size	Each

Showing 10 of 11Show more

Catalog Number Specifications Unit Size Particle Size Diameter (Metric) Length (Metric) Column Format Price (USD)

063710

Full specifications

Each

10 μm

9 mm

50 mm

Loading Column

Request A Quote

Column Format	Loading Column
Diameter (Metric)	9 mm
Flow Rate	0.1 to 10 mL/min
For Use With	ProPac IMAC-10 Column
Length (Metric)	50 mm
Max. Pressure	3000 psi (207 bar)
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Product Line	ProPac
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Loading Column
Unit Size	Each

063272

Full specifications

Each

10 μm

2 mm

50 mm

Analytical Column

Column Format	Analytical Column
Diameter (Metric)	2 mm
Flow Rate	1 mL/min
For Use With	UltiMate 3000 HPLC System
Length (Metric)	50 mm
Max. Pressure	3000 psi (207 bar)
Mobile Phase Compatibility	pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Product Line	ProPac
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Analytical Column
Unit Size	Each

063274

Full specifications

Each

10 μm

2 mm

250 mm

Analytical Column

Request A Quote

Column Format	Analytical Column
Diameter (Metric)	2 mm
Flow Rate	1 mL/min
For Use With	UltiMate 3000 HPLC System
Length (Metric)	250 mm
Max. Pressure	3000 psi (207 bar)
Mobile Phase Compatibility	pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Product Line	ProPac
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Analytical Column
Unit Size	Each

063276

Full specifications

Each

10 μm

4 mm

50 mm

Analytical Column

Column Format	Analytical Column
Diameter (Metric)	4 mm
Flow Rate	1 mL/min
For Use With	UltiMate 3000 HPLC System
Length (Metric)	50 mm
Max. Pressure	3000 psi (207 bar)
Mobile Phase Compatibility	pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Product Line	ProPac
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Analytical Column
Unit Size	Each

063278

Full specifications

Each

10 μm

4 mm

250 mm

Analytical Column

Column Format	Analytical Column
Diameter (Metric)	4 mm
Flow Rate	1 mL/min
For Use With	UltiMate 3000 HPLC System
Length (Metric)	250 mm
Max. Pressure	3000 psi (207 bar)
Mobile Phase Compatibility	pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Product Line	ProPac
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Analytical Column
Unit Size	Each

063280

Full specifications

Each

10 μm

9 mm

250 mm

Analytical Column

Request A Quote

Column Format	Analytical Column
Diameter (Metric)	9 mm
Flow Rate	1 mL/min
For Use With	UltiMate 3000 HPLC System
Length (Metric)	250 mm
Max. Pressure	3000 psi (207 bar)
Mobile Phase Compatibility	pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Product Line	ProPac
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Analytical Column
Unit Size	Each

063282

Full specifications

10 μm

22 mm

250 mm

Loading Column

Request A Quote

Column Format	Loading Column
Diameter (Metric)	22 mm
Flow Rate	0.1 to 10 mL/min
For Use With	UltiMate 3000 HPLC System
Length (Metric)	250 mm
Max. Pressure	3000 psi (207 bar)
Mobile Phase Compatibility	pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Analytical Column
Unit Size	-

063615

Full specifications

Each

10 μm

9 mm

50 mm

Analytical Column

Request A Quote

Column Format	Analytical Column
Diameter (Metric)	9 mm
Flow Rate	1 mL/min
For Use With	UltiMate 3000 HPLC System
Length (Metric)	50 mm
Max. Pressure	3000 psi (207 bar)
Mobile Phase Compatibility	pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Product Line	ProPac
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Analytical Column
Unit Size	Each

063617

Full specifications

Each

10 μm

1 mm

50 mm

Analytical Column

Request A Quote

Column Format	Analytical Column
Diameter (Metric)	1 mm
Flow Rate	1 mL/min
For Use With	UltiMate 3000 HPLC System
Length (Metric)	50 mm
Max. Pressure	3000 psi (207 bar)
Mobile Phase Compatibility	pH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Product Line	ProPac
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Analytical Column
Unit Size	Each

063667

Full specifications

Each

10 μm

4 mm

50 mm

Loading Column

Column Format	Loading Column
Diameter (Metric)	4 mm
Flow Rate	0.1 to 10 mL/min
For Use With	ProPac IMAC-10 Column
Length (Metric)	50 mm
Max. Pressure	3000 psi (207 bar)
Particle Size	10 μm
pH	2 to 12
Pore Size	Nonporous
Temperature	60°C
Stationary Phase	Lysozyne Poly(IDA) Grafts
Product Line	ProPac
Packing Material	Spherical, Nonporous Polymer
Column Type	Separation of Metal-Binding Proteins
Phase	Affinity Separation
Type	Loading Column
Unit Size	Each

Showing 10 of 11Show more

Tailored Specificity for IMAC Separation of Proteins

Retention control by imidazole concentration or pH gradient
Stable between pH 2 to 12, compatible with common protein purification reagents (nonionic detergents, reducing agents)
Effective under native or denaturing HPLC conditions
Wide range of metal-specific applications, including the separation of His-tagged protein aggregation variants, prion peptide analysis and separation of monoclonal antibodies
Shipped free from any metal and ready to be charged with the metal of your choice.
Reusable columns with metal tailored specificity
Resolve target proteins using a single column in a high resolution gradient run
High purity separations of metal-binding proteins

Includes: 10µm, nonporous, polymeric beads coated with a hydrophilic layer, then grafted with poly(IDA) chains.