Centri-Sep™ Spin Columns are used for the fast and efficient purification of nucleic acids. The Centri-Sep™ gel provides excellent recovery of DNA fragments larger than 16 base pairs, while removing greater than 98% of salts, NTPs, and other low molecular weight impurities. Features of Centri-Sep™ columns:
• Processes samples faster than ultrafiltration columns
• Achieves rapid and efficient separation
• Permits use of custom buffers
• Convenient 20–100 µL sample size
• Dry gel format
• Room temperature storage
• Microcentrifuge format
Designed for performance
The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. Each Centri-Sep™ unit consists of a specially fritted microfuge tube, dry gel, a wash tube, and a sample collection tube, all specifically designed for this purpose.
The Centri-Sep™ gel provides excellent recovery of DNA fragments with sizes greater than 16 base pairs or a 25-mer, while removing >98% of salts, NTPs, and other unwanted low molecular weight impurities.
The column gel is hydrated with either reagent-grade water or a buffer of your choice, and spun in a microcentrifuge or swinging-bucket centrifuge to remove the intersititial fluid. Your sample is then applied to the column and spun again, leaving you not only with a sample purified through removal of low molecular weight components, but also exchanged into the buffer of your choice.