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Applied Biosystems™

7500 Fast Real-Time PCR System, laptop, QST

Catalog number: 4365464
Applied Biosystems™

7500 Fast Real-Time PCR System, laptop, QST

Catalog number: 4365464
Catalog Number
Unit Size
1 system
Price (USD)
Catalog NumberUnit SizePrice (USD)
43654641 systemContact Us ›
Product Overview
Citations & References
Additional Information
The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. It is a sophisticated platform for users who require extended capabilities and maximum versatility. The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.
• Powerful, five-color platform is calibrated for the broadest range of dyes available: FAM™⁄SYBR® Green I, VIC®⁄JOE™, NED™⁄ TAMRA™⁄ Cy3™, ROX™⁄Texas Red™, and Cy5™ dyes
• Specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets
• Advanced multi-componenting algorithm minimizes spectral crosstalk – superior for multiplexing
• User-friendly software includes plate set-up wizards, multi-plate data viewing capabilities, and advanced analysis tools to make data processing simple and straightforward

NEW - 7500 Software v2.x
Now the easy-to-use StepOne software is available for both the 7500 and 7500 Fast systems with the 7500 Software v2.x upgrade. The 7500 Software v2.x incorporates your favorite StepOne Software features, such as multiple plate setup wizards, calculators for standard curve dilution and master mix recipes, QC flags, data filters, and email notification when a run is finished. The 7500 Software v2.x also includes an enhanced Gene Expression Study package and has a variety of new melting curve protocol options, including multiple peak detection, step and hold temperature control, and customizable ramp rates.

The NEW Gene Expression Study package accommodates large studies better than any other instrument software package:
• Import an unlimited number of Comparative CT (relative quantitation) files to one study
• Group samples and view data both by technical replicate group and biological replicate group
• Use any gene(s) as an endogenous control, including averaging multiple controls together
• Enter known efficiency values to be factored into the RQ results

21 CFR Part 11 Module available
The SDS v1.4 21CFRp11 Module is a powerful tool for assisting with 21CFRp11 compliance, while still offering the flexibility of user-customizable configuration settings.
• Individual user log-ins can be added for up to four user groups, each group with designated permission settings
• User-customizable permission settings include fourteen system activities, e-signature authority designation, and additional security settings to give you maximum control over your compliance efforts
• Audit trails can be enabled or disabled depending on your traceability needs
• A selection of e-signatures is available to ease e-signatures into your workflow

Supports Many Applications
Applications include gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +⁄– assays utilizing internal positive controls. To facilitate many of these applications, we provide preformulated, ready-to-use, quality-tested, TaqMan® assays for use with the 7500 system. Now you can reduce your assay optimization efforts.

Upgrade to High-Speed Thermal Cycling
An optional upgrade to a the 7500 Fast System is available. This 7500 Fast System uses our master mix formulations and enables you to shorten your real-time PCR runs to as little as 30 minutes.

For Research Use Only. Not for use in diagnostics procedures.


For Use With (Equipment)
7500 Fast System
Reaction Speed
Fast, Standard
Dynamic Range
9 logs of linear dynamic range
Down to 1 copy of RNase P gene from human genomic DNA
Data Acquisition
Collects data for all 5 filters for all wells regardless of plate setup
Calibrated Dye
SYBR™ Green I, ROX™, NED™, TAMRA™, JOE™, Texas Red™, Cy5®, VIC™, FAM™, Cy3®
34 cm (W) x 49 cm (H) x 45 cm (D) (13.39 in x 19.29 in x 17.72 in)
External Computer
96-well plate
High-throughput Compatibility
High-throughput Compatible, Multiplexing
Passive Reference Dye
ROX (Separate Tube), ROX (Pre-mixed)
Tungsten-halogen lamp, five-emission filters and CCD camera, Five-excitation filters
Max. Power
950 W
Can distinguish between 5000 and 10000 template copies with 99.7% confidence
Reaction Volume Range
10-30 µl
Sample Ramp Rate
Fast mode: ± 3.5°C⁄sec, Standard mode: ± 1.6°C⁄sec
Software Compatibility
Primer Express Software, System Software
Temperature Range (Metric)
Temperature Uniformity
Within 1.0°C (30 seconds after clock start)
Temperature Accuracy
Within 0.5°C (35-95°C) of setpoint⁄display temp (3 min. after start)
Manual loading of four-five 96-well plates per 8-hr day (real-time throughput)
Thermal Cycling System
Peltier-Based System
75 lbs