Centri-Sep™ 96's hydrated matrix enables effective and reliable high-throughput removal of excess BigDye® terminators from DNA sequencing reactions, giving you clean sequencing reads with uniform signal intensity starting with the first base. The plate's 96-well format enables you to purify 96 samples simultaneously, either manually or by robotics, within 15 minutes.
• Processed faster than ultrafiltration columns
• Rapid and efficient separations
• Buffer is not preselected
• Convenient 20-100 µL sample size
• Dry gel format
• Room Temperature Storage
• Microcentrifuge format
Designed for Performance
The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. Each Centri-Sep unit consists of a specially fritted microfuge tube, dry gel, a wash tube and a sample collection tube, all specifically designed for this purpose.
The Centri-Sep™ gel will provide excellent recovery of DNA fragments with sizes greater than 16 base pairs or 25-mer, while removing 98% of salts, NTP's and other unwanted low molecular-weight impurities.
The column gel is hydrated with either reagent-grade water or a suitable buffer or your choice, and spun in a microcentrifuge or swinging-bucket centrifuge to remove the intersititial fluid. Your sample is then applied to the column and spun again, leaving you not only with a sample purified through removal of low-molecular-weight components, but also exchanged into the buffer of your choice.
For Research Use Only. Not for use in diagnostics procedures.