Invitrogen™

MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit, compatible with Tempus™ Blood RNA Tubes

Catalog number: 4451893
Invitrogen™

MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit, compatible with Tempus™ Blood RNA Tubes

Catalog number: 4451893
Catalog Number
4451893
Unit Size
96 preps
Price (USD)
Price: 730.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
445189396 preps
Price: 730.00
Your Price:
-
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The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit purifies total RNA (including miRNA) from Tempus™ Blood RNA Tubes using proven magnetic bead-based technology. A TURBO DNase™ step efficiently eliminates contaminating gDNA ensuring the highest purity RNA possible suitable for real-time RT-PCR and microarray analysis.

• Flexible purification format—use microfuge tubes, manual 96-well plates, or open-format automated 96-well plates from the same kit
• Simple magnetic bead workflow—no spin columns
• Total RNA without RNase activity, contaminating gDNA, or RT-PCR inhibitors

Maximize Total RNA Yields From Blood Samples
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit utilizes proven magnetic bead-based technology to purify maximal yields of total RNA from Tempus™ Blood RNA Tubes. The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can recover 3-25 µg from a single Tempus™ Blood RNA tube. A TURBO DNase™ step efficiently eliminates contaminating gDNA that can interfere with downstream applications and ensures the highest purity RNA possible.

The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can also detect miRNA from Tempus™ Blood RNA tubes as shown by TaqMan® gene expression assays. Users are strongly advised to perform appropriate validation studies against their miRNA targets.

Flexible Purification Formats
The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be processed in low-, medium- or high-throughput formats with superior workflow and handling compared to traditional competitive methods.
• For low-throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be used in individual 1.5mL microfuge tubes with the bead?RNA-complex remaining in a single processing tube until the final elution step.
• For medium-to-high–throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be done in a 96-well processing plate with either a single or multi-channel pipettor to accelerate the purification process.
• For high-throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is compatible with manual and automated 96-well processing. Simply process multiple batches of tubes in series or in parallel, through the up-front centrifugation process, to the convenient stopping point, until enough samples can be loaded onto a 96-well processing plate for the RNA purification steps. The RNA purification process can be handled on either open-format liquid handlers, in conjunction with a 96-well magnetic stand on the deck, or magnetic bead movers like the MagMAX™ Express-96 Magnetic Particle Processor (deep well version only).

Rapid Processing Time
Processing times will vary based on format selected, but twelve Tempus™ Blood RNA tubes can be processed manually with the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit in approximately 2 hours.

An Alternative to Full Purification
For users who perform primarily TaqMan®-based real-time RT-PCR experiments, and do not need to archive RNA⁄cDNA, an alternative to the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR. This kit makes it possible to perform expression analysis directly from Tempus™ Blood RNA tubes without conventional RNA purification.

One-Stop Shopping
MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is compatible with our suite of reverse transcription mixes (SuperScript®), PCR master mixes (both TaqMan® and SYBR® based), and other blood workflow products. Simplify the ordering process and reduce freight cost by getting more from one supplier.

Specifications:
• Yield (Range): 3 - 25µg
• Quality (RIN) ): ≥ 7
• Quality (A260⁄280) ): 1.8 – 2.2
• gDNA (w⁄w) ): <1.56%
• Equipment Needed :
     *Refrigerated centrifuge (4°C) with swing bucket rotor capable of 5,000 x g with
     *50 mL conical tube adapters
     *50ml conical tubes
     *For tube format users: Tabletop microcentrifuge or picocentrifuge
• Number of Preps: 96 preparations (blood tubes)

NOTE: Blood tubes and magnetic stands are sold separately.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Specifications

For Use With (Application)
Real-Time Quantitative PCR (qPCR)
No. of Reactions
96 Prep
Format
Tube(s), 96-well plate
Yield
25 µg
High-throughput Compatibility
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
Product Line
Ambion™, MagMAX™
Final Product Type
Total RNA
Purification Target
Total RNA
Sample Type
Blood
Purification Time
1.9 hrs
Isolation Technology
Magnetic Bead

Contents & Storage

Kit Components
Box 1:
Store at RT

• Tempus™ Resuspension Solution, 12 mL
• Binding Solution Concentrate 5 mL
• Wash Solution 1 Concentrate, 2 x 16mL (Before using the Wash Solution 1 Concentrate, add the correct volume of isopropanol (user-supplied), as indicated on the bottle)
• Wash Solution 2 Concentrate, 30 mL (Before using the Wash Solution 2 Concentrate, add the correct volume of ethanol (user-supplied), as indicated on the bottle)
• Elution Buffer 10 mL
• Processing Plate (96-well) 1 plate

Store at 2 to 8 °C, Do not freeze.
• Tempus™ Pre-Digestion Wash, 2 x 200 mL
• RNA Binding Beads, 2 mL
• Tempus™ 1X PBS, 2 x 150 mL

Box 2:
Store at -15 to -25 °C
• Tempus™ Proteinase, 0.25 mL
• TURBO™ DNase, 1 mL