Applied Biosystems™

TaqMan™ Protein Assays Open Kit

Green features
Catalog number: 4453745
Applied Biosystems™

TaqMan™ Protein Assays Open Kit

Green features
Catalog number: 4453745
Catalog Number
4453745
Unit Size
4,000 reactions
Price (USD)
Price: 1,380.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
44537454,000 reactions
Price: 1,380.00
Your Price:
-
Product Overview
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The TaqMan® Protein Assays Open Kit contains the TaqMan® Protein Assays Oligo Probe Kit (4448549) and the TaqMan® Protein Assays Buffer Kit (4448571) required for making TaqMan® Protein Assay probes, along with the customer-supplied biotinylated antibodies. You supply the biotinylated antibodies and add them to streptavidin-oligonucleotides (prox-oligos) to make the 2 antibody-oligonucleotide assay probes utilized in binding. The resulting assay probes are utilized to help quantitate protein expression using gold standard TaqMan® 5' nuclease chemistry with a workflow and sample quantity similar to our TaqMan® Gene Expression and MicroRNA Assays. As the protein expression results are obtained on the same analytical platform, these revolutionary assays enable direct correlation of mRNA and⁄or miRNA expression to protein expression.

Key product features:
• Small Sample Size – use up to 500 times less sample input compared to traditional methods
• Sensitive Detection – directly measure in vitro protein-protein interactions with only a few thousand cells
• Revolutionary – directly correlate mRNA and⁄or microRNA to protein
• Simple Workflow – 1-step lysis sample preparation, universal protocol
• Quantitative – protein marker expression fold changes detected

Simple Workflow
You make TaqMan® Assay probes by using your biotinylated antibodies. Then follow the same workflow used with our pre-designed assays: the sample prep step consists of a gentle, 1-step cell lysis using a buffered non-ionic detergent, with no further sample clean up or purification required. The crude lysate samples can be directly mixed with the paired assay probes from any Protein Assay Kit for the target binding step. The entire experimental process, from sample prep to data output, can be completed in about 5 hours (Figure 1).

For Research Use Only. Not for use in diagnostics procedures.

Specifications

For Use With (Equipment)
7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOnePlus™, Fast Mode
Green Features
Sustainable packaging
Label or Dye
FAM
No. of Tests
4000 Reactions
Passive Reference Dye
ROX (Pre-mixed)
Primer Probe Compatibility
TaqMan™
Product Line
TaqMan™
Product Type
Protein Assays Open Kit
Target Specificity
Not Target-Specific
Detection Method
Primer-Probe Detection
GC-Rich PCR Performance
Low
Hot Start
No
No. of Reactions
4000 Reactions
PCR Method
qPCR Protein Quantification
Reaction Speed
Fast
Sample Type
Tissue, Cells
Technique
Proximity Ligation Assay

Contents & Storage

The TaqMan™ Protein Assays Open Kit contains the TaqMan™ Protein Assays Oligo Probe Kit (4448549) and the TaqMan™ Protein Assays Buffer Kit (4448571)for making TaqMan™ Protein Assay probes.