Thermo Scientific™

SMART Digest™ ImmunoAffinity (IA) Protein A Kits

Catalog number: 60111-104
Thermo Scientific™

SMART Digest™ ImmunoAffinity (IA) Protein A Kits

Catalog number: 60111-104

Obtain fast, simple, highly reproducible, and sensitive immunocapture and protein digestion in a single well using Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Protein A Kits. These kits were designed to remove the challenges often associated with the process of biomarker quantitation by providing a simple and easy capture-digestion workflow that takes only hours instead of overnight. The SMART Digest IA Protein A Kits target purification of immunoglobulins due to the strong affinity for binding to the Fc portion of the molecule.

 
Catalog Number
60111-104
Unit Size
Each
Description
SMART Digest IA Kit, Protein A magnetic
Price (USD)
Full specifications
TypeImmunocapture + Protein Digestion combined on a single bead
DescriptionSMART Digest IA Kit, Protein A magnetic
IncludesMagnetic SMART Digest Resin
Product LineSMART Digest
For Use With (Application)Peptide Quantitation
FormatSMART Digest Resin, Magnetic
EnzymeTrypsin
CaptureProtein A
CleavageC-terminal side of lysine and arginine amino acids
Unit SizeEach
Catalog Number
Unit Size
Each
Description
SMART Digest IA Kit, Protein A non-magnetic
Price (USD)
Full specifications
TypeImmunocapture + Protein Digestion combined on a single bead
DescriptionSMART Digest IA Kit, Protein A non-magnetic
IncludesNon-magnetic SMART Digest Resin
Product LineSMART Digest
For Use With (Application)Peptide Quantitation
FormatSMART Digest Resin, Non-magnetic
EnzymeTrypsin
CaptureProtein A
CleavageC-terminal side of lysine and arginine amino acids
Unit SizeEach
Catalog Number
Unit Size
Each
Description
SMART Digest IA Kit, Protein A non-magnetic with SOLAµ SPE and collection plate
Price (USD)
Full specifications
TypeImmunocapture + Protein Digestion combined on a single bead
DescriptionSMART Digest IA Kit, Protein A non-magnetic with SOLAµ SPE and collection plate
IncludesNon-magnetic SMART Digest Resin, Collection Plate, SOLAµ SPE Plate
Product LineSMART Digest
For Use With (Application)Peptide Quantitation
FormatSMART Digest Resin, Non-magnetic
EnzymeTrypsin
CaptureProtein A
CleavageC-terminal side of lysine and arginine amino acids
Unit SizeEach
Catalog Number
Unit Size
Each
Description
SMART Digest IA Kit, Protein A magnetic with SOLAµ SPE and collection plate
Price (USD)
Full specifications
TypeImmunocapture + Protein Digestion combined on a single bead
DescriptionSMART Digest IA Kit, Protein A magnetic with SOLAµ SPE and collection plate
IncludesMagnetic SMART Digest Resin, Collection Plate, SOLAµ SPE Plate
Product LineSMART Digest
For Use With (Application)Peptide Quantitation
FormatSMART Digest Resin, Magnetic
EnzymeTrypsin
CaptureProtein A
CleavageC-terminal side of lysine and arginine amino acids
Unit SizeEach
Showing 4 of 4
Catalog NumberSpecificationsUnit SizeDescriptionPrice (USD)
60111-104Full specifications
EachSMART Digest IA Kit, Protein A magneticContact Us ›
TypeImmunocapture + Protein Digestion combined on a single bead
DescriptionSMART Digest IA Kit, Protein A magnetic
IncludesMagnetic SMART Digest Resin
Product LineSMART Digest
For Use With (Application)Peptide Quantitation
FormatSMART Digest Resin, Magnetic
EnzymeTrypsin
CaptureProtein A
CleavageC-terminal side of lysine and arginine amino acids
Unit SizeEach
60111-101Full specifications
EachSMART Digest IA Kit, Protein A non-magneticContact Us ›
TypeImmunocapture + Protein Digestion combined on a single bead
DescriptionSMART Digest IA Kit, Protein A non-magnetic
IncludesNon-magnetic SMART Digest Resin
Product LineSMART Digest
For Use With (Application)Peptide Quantitation
FormatSMART Digest Resin, Non-magnetic
EnzymeTrypsin
CaptureProtein A
CleavageC-terminal side of lysine and arginine amino acids
Unit SizeEach
60111-102Full specifications
Each SMART Digest IA Kit, Protein A non-magnetic with SOLAµ SPE and collection plateContact Us ›
TypeImmunocapture + Protein Digestion combined on a single bead
DescriptionSMART Digest IA Kit, Protein A non-magnetic with SOLAµ SPE and collection plate
IncludesNon-magnetic SMART Digest Resin, Collection Plate, SOLAµ SPE Plate
Product LineSMART Digest
For Use With (Application)Peptide Quantitation
FormatSMART Digest Resin, Non-magnetic
EnzymeTrypsin
CaptureProtein A
CleavageC-terminal side of lysine and arginine amino acids
Unit SizeEach
60111-103Full specifications
EachSMART Digest IA Kit, Protein A magnetic with SOLAµ SPE and collection plateContact Us ›
TypeImmunocapture + Protein Digestion combined on a single bead
DescriptionSMART Digest IA Kit, Protein A magnetic with SOLAµ SPE and collection plate
IncludesMagnetic SMART Digest Resin, Collection Plate, SOLAµ SPE Plate
Product LineSMART Digest
For Use With (Application)Peptide Quantitation
FormatSMART Digest Resin, Magnetic
EnzymeTrypsin
CaptureProtein A
CleavageC-terminal side of lysine and arginine amino acids
Unit SizeEach
Showing 4 of 4
Solving the challenges of low-level quantitation in complex matrices

The need to identify low-level biomarkers is forcing the analytical community to deal with extremely high levels of analyte and sample complexity, which makes quantitation challenging.

As these proteins are often present at low levels in complex biological matrices, an immunoaffinity capture step is typically employed to purify the sample and increase sensitivity. This step is often followed by protein digestion. Current processes are often time consuming, multistep, and prone to irreproducibility.

Immunocapture and Protein Digestion in a Single Well

These IA kits deliver a process that is:

  • Fast
  • Simple
  • Highly reproducible
  • Sensitive
Unique Design Reduces Workflow to Hours

In SMART Digest IA Kits, the immunocapture reagents and the temperature-activated, thermally stable trypsin are co-immobilized onto a resin. Following enrichment, the enzyme is activated at elevated temperatures for accelerated digestion under protein denaturing conditions, resulting in a workflow which takes hours rather than overnight.