The PrioCHECK® FMDV NS ELISA is an ELISA that detects antibodies against the highly conserved non-structural (NS) protein of the FMD virus. The test can therefore be used for all species.
The ELISA test plates of the kit contain the FMDV nonstructural protein, 3ABC, captured by the coated anti-3ABC monoclonal antibody (mAb). A second mAb labeled with an enzyme that generates a color signal (the detection antibody) is then added. The reaction between the FMDV-NS antigen and the detection antibody is blocked by antibodies directed against the non-structural proteins present in the sample. The signal is then measured and when no color is formed, the antibodies in the sample have competed for the viral protein coated on the plate. Samples with a percent inhibition (PI) value of ≥ 50% have shown an immune response to the foot and mouth disease virus. With this result, the sample is immuno-reactive (i.e., anti-FMDV antibodies are present).