Thermo Scientific™ Pierce™ Protein A/G Magnetic Agarose Beads provide a fast, convenient method for purification of immunoglobulins from serum, cell culture supernatant, or ascites. For antibody purification, the beads are incubated with the antibody sample and then magnetically separated from the supernatant. Nonspecifically bound serum or host cell protein can be washed away before dissociating bound antibody with elution buffer. The beads are removed from the solution manually using a magnetic stand or by automation using an instrument such as the Thermo Scientific™ KingFisher™ Flex Magnetic Particle Processor. Automated instruments are especially useful for higher throughput purification and screening of purification conditions.Download the KingFisher™ Duo BindIt™ Software protocol (.bdz) >Download the KingFisher™ Flex BindIt™ Software protocol (.bdz) >
Pierce Protein A/G Magnetic Agarose Beads contain a 50.5 kDa Protein A/G recombinant fusion protein that is covalently attached to a magnetite-embedded agarose core particle. These beads are not simply a mixed immobilization of separate Protein A and Protein G polypeptides, nor are they a mixture of Protein A magnetic beads and Protein G magnetic beads. The recombinant chimeric Protein A/G combines four IgG binding domains from Protein A and two binding domains from Protein G, making it a more general and convenient tool for purifying immunoglobulins.
Features of Protein A/G Magnetic Agarose beads:
• Protein A/G – immobilized recombinant fusion protein of the antibody-binding domains of Protein A and Protein G enables IgG purification from nearly any mammalian species
• High capacity—sufficient for both routine and demanding purification procedures
• Low non-specific binding—optimized purification protocol results in better IgG purification
• Flexibility—convenience of IgG binding domains of both Protein A and Protein G on one bead
• Compatibility— beads are compatible with manual and automated applications
• Inert and stable—superior manufacturing method immobilizes Protein A/G by leach-resistant covalent bonds
The high-performance, magnetite-containing, superparamagnetic magnetic agarose beads are validated and optimized for use with high-throughput magnetic platforms, such as the KingFisher 96 and KingFisher Flex magnetic particle processors, but the beads also enable premium performance for simple benchtop purification applications using an appropriate magnetic stand.
Our recombinant Protein A/G binds to all human IgG subclasses, making it the ideal choice for purification of polyclonal or monoclonal IgG antibodies whose subclass identities have not been determined. In addition, it binds to IgA, IgE, IgM and (to a lesser extent) IgD. Protein A/G binds well to all mouse IgG subclasses but does not bind mouse IgA, IgM, or serum albumin. This makes Protein A/G an excellent tool for purification and detection of mouse monoclonal antibodies from IgG subclasses, without interference from IgA, IgM, and murine serum albumin. Individual subclasses of mouse monoclonals are likely to have a stronger affinity to the chimeric Protein A/G than to either Protein A or Protein G.