Informational:This kit contains a rabbit Tb-anti-GST antibody—an equivalent kit containing a goat-sourced antibody is also available.

LanthaScreen™ TR-FRET ERR gamma Coactivator Assay Kit, rabbit

Catalog number: A15121

LanthaScreen™ TR-FRET ERR gamma Coactivator Assay Kit, rabbit

Catalog number: A15121
Catalog Number
A15121
Unit Size
800 x 20 µL assays
Price (USD)
Catalog NumberUnit SizePrice (USD)
A15121800 x 20 µL assaysContact Us ›
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This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4408:

The LanthaScreen® TR-FRET Estrogen Related Receptor (ERR) gamma Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential ERR gamma ligands as antagonists/inverse agonists of coactivator displacement. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide (Figure 1).

How it works

The LanthaScreen® TR-FRET ERR gamma Coactivator Assay Kit includes a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human ERR gamma ligand-binding domain (ERR gamma-LBD) that is tagged with glutathione-S-transferase (GST). To assay, ERR gamma-LBD is added to test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti- GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound.

Contents and Storage:

The LanthaScreen® TR-FRET ERR gamma Coactivator Assay Kit contains ERR gamma-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Category
Biochemical Assay
Color
Green
Detection Method
Fluorescent
Druggable Target
Nuclear Receptors
For Use With (Equipment)
Microplate Reader
Format
384-well plate
Label or Dye
Tb (Terbium)
Product Line
LanthaScreen®
Product Size
800 x 20 µL assays
Readout
TR-FRET (Activity)
Technique
TR-FRET
Validated Application
Nuclear Receptor Assay

Contents & Storage

Tb-anti-GST Antibody (Rabbit): Store at -20 °C
ERR gamma-LBD, GST: Store at -80 °C
Fluoroscein Coactivator Peptide: Store at -20 °C
TR-FRET Coregulatore Buffer B: Store at 4 °C
1M DTT: Store at -20 °C or -80 °C