The Applied Biosystems TaqMan Advanced Serum/Plasma 96-Well Plates, Standard, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well standard plate format. This product contains a set of 2 plates for profiling of up to 188 unique miRNAs in serum/plasma samples, and includes endogenous and exogenous miRNA controls for normalization of data results.
Features of the TaqMan Advanced Serum/Plasma 96-Well Plates include:
—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
• Sample savings
—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
• Updated content
—assay content updated for mirBase v.21
• Easy to use
—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
• Gold-standard TaqMan qPCR chemistry
—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA familiesProduct details:Number of plates:
2Number of unique miRNAs (not including exogenous controls):
cel-miR-39-3p, ath-miR159aEndogenous control:
hsa-miR-16-5pCompatible with the following Applied Biosystems qPCR instruments:
Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k FlexRecommended master mix: TaqMan Fast Advanced Master MixNotes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit
, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.