Amine-reactive Thermo Scientific TMT10plex Isobaric Mass Tag Labeling Reagent Set plus TMT11-131C increases sample multiplexing from 10-plex to 11-plex, enabling even greater throughput for protein identification and quantitative analysis by tandem mass spectrometry (MS).
Features of this TMT10plex Isobaric Label Reagent Set plus TMT11-131C:
—concurrent MS analysis of up to 11 samples derived from cells, tissues, or biological fluids
—increased multiplex capability results in fewer missing quantitative values among samples and higher confidence among replicates
—amine-reactive, NHS-ester-activated reagents ensure efficient labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity
—optimized for use with high resolution Thermo Scientific MS/MS platforms, such as the Q Exactive, Orbitrap Elite, and Orbitrap Fusion Tribrid instruments with data analysis fully supported by Proteome Discoverer 2.1
The Tandem Mass Tag (TMT) reagents are specially designed to enable identification and quantitation of proteins in different samples using tandem mass spectrometry. The TMT11-131C tag structure is identical to the other TMT10pex tags, but the TMT11-131C mass reporter is labeled with only 13C isotopes. The addition of the TMT11-131C tag enables separation of the 131 mass reporter ion of the original TMT10plex reagents into N and C variants, creating an 11th channel for relative quantitation using high resolution Orbitrap MS instruments.
Advantages of the TMT label reagents include increased multiplex relative quantitation, increased sample throughput, and fewer missing quantitative values among samples. TMT10plex label reagents plus TMT11-131C is ideal for analysis of multiple protein samples from inhibitor dose response experiments, time course experiments, or biological replicates.
TMT11-131C is available as a standalone tag (Cat. No. A34807), or as part of this TMT10plex Isobaric Label Reagent Set plus TMT11-131C. When combined with the industry-leading, high resolution Orbitrap instruments and software, TMT reagents provide integrated total solutions for quantitative protein expression analysis.
These products are subject to a limited use label license. Cell Signaling Technologies, IQ Proteomics, and OmicScouts are authorized service providers for tandem mass tags.Related productsTMT10plex Isobaric Label Reagent Set, 8 x 0.2 mgTMT11-131C, 1 x 5 mgTMT10plex Isobaric Label Reagent Set, 1 x 5 mg
For high resolution analysis of the TMT-labeled peptides, the recommended LC column for Nanospray Flex source is Acclaim PepMap 100 C18 LC Column
(Cat. No. 164942 or 164939). For EASY-Spray source, the recommended LC column is EASY-Spray C18 LC Column
(Cat. No. ES802 or ES803).