Invitrogen™

RNAqueous™ Total RNA Isolation Kit

Catalog number: AM1912
Invitrogen™

RNAqueous™ Total RNA Isolation Kit

Catalog number: AM1912
Catalog Number
AM1912
Unit Size
50 preps
Price (USD)
Price: 378.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM191250 preps
Price: 378.00
Your Price:
-
Product Overview
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Additional Information
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The RNAqueous® Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 102–107 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The RNAqueous® Kit provides:

• Convenient syringe-based filtration
• Compatibility with vacuum manifolds when processing multiple samples

How it works
The RNAqueous® Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov

Specifications

For Use With (Application)
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction
Product Type
Kit
Format
Syringe Filter
Includes
6mL Formaldehyde Load Dye; 60mL Lysis/Binding Solution; 60mL Water for 64% Ethanol; 4mL Lithium Chloride Precipitation Solution; 40mL Wash Solution 1; 80mL Wash Solution 2/3 Concentrate; 50 Filter Cartridges; 100 Collection Tubes; 10mL Elution Solution
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Shipping Condition
Room Temperature
Product Line
Ambion™, RNAqueous™
Final Product Type
Total RNA
Purification Target
Total RNA
Sample Type
Plant Samples, Viral Samples, Tissue, Yeast, Bacteria, Cells
Isolation Technology
Spin Column (Glass Fiber Filter)

Contents & Storage

• 6 ml Formaldehyde Load Dye (-20°C)
• 60 ml Lysis/Binding Solution (4°C)
• 60 ml Water for 64% Ethanol (4°C)
• 4 ml Lithium Chloride Precipitation Solution (4°C)
• 40 ml Wash Solution 1 (4°C)
• 80 ml Wash Solution 2/3 Concentrate (4°C)
• 50 Filter Cartridges (room temperature)
• 100 Collection Tubes (room temperature)
• 10 ml Elution Solution (-20°C, 4°C, or room temperature)