Invitrogen™

Novex™ pH 3-7 IEF Protein Gels, 1.0 mm

Catalog number: EC6645BOX
Invitrogen™

Novex™ pH 3-7 IEF Protein Gels, 1.0 mm

Catalog number: EC6645BOX
Novex™ pH 3-7 IEF Gels are used for pI determination and are excellent for native applications using soluble proteins. Isoelectric focusing (IEF) is an electrophoresis technique that separates proteins based on their isoelectric point (pI). The pI is the pH at which a protein has no net charge and does not move in an electric field. Vertical IEF gels effectively create a pH gradient so proteins separate according to their unique pI.
 
Catalog Number
EC6645BOX
Unit Size
5 gels (1 box)
Wells
10-well
Price (USD)
Price: 230.00
Your Price:
Availability
-
Full specifications
Quantity
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage5%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeIEF
Sample Loading Volume25 µL
Separation RangepH 3.5 to 6.8
Separation TypeIsoelectric Focusing
Shelf Life6 Months
Wells10-well
Mode of SeparationpI (Charge)
Product LineNovex™
Quantity5 Gels/Box
Shipping ConditionWet Ice
Storage RequirementsStore at 2°C to 8°C. Do not freeze.
Width (Metric)8 cm
Unit Size5 gels (1 box)
Catalog Number
Unit Size
5 gels (1 box)
Wells
12-well
Price (USD)
Price: 230.00
Your Price:
Availability
-
Full specifications
Quantity
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage5%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeIEF
Sample Loading Volume20 µL
Separation RangepH 3.5 to 6.8
Separation TypeIsoelectric Focusing
Shelf Life6 Months
Wells12-well
Mode of SeparationpI (Charge)
Product LineNovex™
Quantity5 Gels/Box
Shipping ConditionWet Ice
Storage RequirementsStore at 2°C to 8°C. Do not freeze.
Width (Metric)8 cm
Unit Size5 gels (1 box)
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeWellsPrice (USD)AvailabilityQuantity
EC6645BOXFull specifications
5 gels (1 box)10-well
Price: 230.00
Your Price:
-
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage5%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeIEF
Sample Loading Volume25 µL
Separation RangepH 3.5 to 6.8
Separation TypeIsoelectric Focusing
Shelf Life6 Months
Wells10-well
Mode of SeparationpI (Charge)
Product LineNovex™
Quantity5 Gels/Box
Shipping ConditionWet Ice
Storage RequirementsStore at 2°C to 8°C. Do not freeze.
Width (Metric)8 cm
Unit Size5 gels (1 box)
EC66452BOXFull specifications
5 gels (1 box)12-well
Price: 230.00
Your Price:
-
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage5%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeIEF
Sample Loading Volume20 µL
Separation RangepH 3.5 to 6.8
Separation TypeIsoelectric Focusing
Shelf Life6 Months
Wells12-well
Mode of SeparationpI (Charge)
Product LineNovex™
Quantity5 Gels/Box
Shipping ConditionWet Ice
Storage RequirementsStore at 2°C to 8°C. Do not freeze.
Width (Metric)8 cm
Unit Size5 gels (1 box)
Showing 2 of 2

IEG gels can be used to readily detect minor changes in a protein due to deamination, phosphorylation, or glycosylation, and can resolve different proteins of similar size that cannot be resolved on standard SDS-PAGE gels. Pre-focusing is not required. Total run time is approximately 2.5 hours.

Formulation
Novex™ IEF gels are 5% polyacrylamide and consist of high-purity acrylamide, bisacrylamide, TEMED, APS, ultrapure water, and 2% ampholytes. They do not contain denaturing reagents.

Recommended buffers
IEF is a sensitive technique which is affected by many factors. Our optimized, premixed IEF buffers reduce variability and help you obtain consistent results.