Thermo Scientific™

GeneBLAzer™ TR beta DA Assay Kit

Catalog number: K1389
Thermo Scientific™

GeneBLAzer™ TR beta DA Assay Kit

Catalog number: K1389
Catalog Number
Unit Size
1 plate
Price (USD)
Price: 1,172.00
Your Price:
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K13891 plate
Price: 1,172.00
Your Price:
Product Overview
Citations & References
Additional Information
GeneBLAzer®TR beta DA(Division Arrested) cells and TR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Thyroid hormone receptor beta (TR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UASbla HEK 293T cell line. GeneBLAzer®UAS-bla HEK 293T cells stably express a betalactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-TR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both TR beta DA cells and TR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of T3 Thryoid hormone. In addition, TR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.


Alternate Agonists
9-cis-retinoic acid, AM580
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Division-Arrested Cells
Detection Method
Gene ID (Entrez)
Gene Symbol
Primary Agonist
All-trans-retinoic acid (ATRA)
Product Line
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
T3RB, TR beta, THRB, NR1A2
Cell Line
293T (HEK)
System Type
Druggable Target
Nuclear Receptors

Contents & Storage

Shipping: Dry IceStorage: -80°C/Liquid NitrogenContains sufficient division-arrested cells and substrate to assay one 384-well plate includes:TR beta HEK 293T DA cells (K1389A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg