Thermo Scientific™

GeneBLAzer™ PPAR delta DA Assay Kit

Catalog number: K1395
Thermo Scientific™

GeneBLAzer™ PPAR delta DA Assay Kit

Catalog number: K1395
Catalog Number
Unit Size
1 plate
Price (USD)
Price: 1,264.00
Your Price:
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K13951 plate
Price: 1,264.00
Your Price:
Product Overview
Citations & References
Additional Information
GeneBLAzer® PPAR delta DA (Division Arrested) cells and PPAR delta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-delta (PPAR delta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer® UAS-bla HEK 293T cell line. GeneBLAzer® UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-PPAR delta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both PPAR delta DA cells and PPAR delta-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of L-165041. In addition, PPAR delta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.


Alternate Agonists
Estrone, Genistein, Diethylstibestrol, Ethynylestradiol
4-OH-tam+0.2 nM E2, Tam+0.2 nM E2, 4-OH-tamoxifen, Tamoxifen, RU486+0.2 nM E2, RU486
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Division-Arrested Cells
Detection Method
Gene ID (Entrez)
Gene Symbol
Primary Agonist
Product Line
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
PPAR delta, NR1C2, PPARD
Cell Line
293T (HEK)
System Type
Druggable Target
Nuclear Receptors

Contents & Storage

Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:PPAR delta HEK 293T DA cells (K1395A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg