Thermo Scientific™

GeneBLAzer™ RAR-beta DA Assay Kit

Catalog number: K1407
Thermo Scientific™

GeneBLAzer™ RAR-beta DA Assay Kit

Catalog number: K1407
Catalog Number
K1407
Unit Size
1 plate
Price (USD)
Price: 1,212.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K14071 plate
Price: 1,212.00
Your Price:
-
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GeneBLAzer®RAR beta DA (Division Arrested) cells and RAR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor beta (RAR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293T cell line. GeneBLAzer®UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RAR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both RAR beta DA cells and RAR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of all-trans retinoic acid (ATRA). In addition, RAR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.

Specifications

Alternate Agonists
Docosahexaenoic Acid, Phytanic Acid, All-trans-retinoic acid
Antagonists
none
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Division-Arrested Cells
Detection Method
Fluorescent
Gene Symbol
RARβ
Primary Agonist
9-cis-retinoic acid
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
RAR beta, NR1B2, RARB
Cell Line
293T (HEK)
System Type
GeneBLAzer™
Druggable Target
Nuclear Receptors

Contents & Storage

Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:RAR beta HEK 293T DA cells (K1407A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg