The Tango™ EDG4-bla U2OS cells contain the human Endothelial Differentiation Gene 4 (EDG4) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ EDG4-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of LPA (18:1). In addition, Tango™ EDG4-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.