PureLink® Total RNA Blood Kit allows purification of high-quality total RNA from 50 - 500 µl fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol.
• RNA purification from whole blood in under an hour
• Yields high-quality RNA devoid of non-nucleated erythrocytesSimple RNA Purification from Whole Blood
PureLink® Total RNA Blood Kit is designed for purifying from 50 to 500 µl fresh whole blood or approximately 3.5 x 106
leukocytes (white blood cells) in less than 1 hour. The entire protocol can be done in spin tubes and spin columns. Leukocyte Enrichment for Improved RNA Purification
Whole blood consists of non-nucleated erythrocytes (red blood cells, 4 - 6 million/mm3
) and leukocytes, with the latter present in the minority (5000 - 7000/mm3
). Purifying total RNA from whole blood without the removal of erythrocytes results in low RNA yields and clogging of purification columns. The distinct feature of the PureLink® Total RNA Blood Kit is that it employs a leukocyte enrichment step. Hypotonic shock is used to selectively lyse erythrocytes while not affecting leukocytes. Non-nucleated erythrocytes are efficiently depleted, thus significantly reducing cell debris that may otherwise interfere with RNA purification and other downstream applications. Consequently, yields of high-quality total RNA are obtained using the PureLink® Total RNA Blood Kit. Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:
• Northern Blotting Kits & Reagents
• Nuclease protection assays
• cDNA synthesis
• Real-Time PCR (qPCR)
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.