Invitrogen™

PureLink™ Total RNA Blood Kit

Catalog number: K156001
Invitrogen™

PureLink™ Total RNA Blood Kit

Catalog number: K156001
Catalog Number
K156001
Unit Size
50 preps
Price (USD)
Price: 406.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K15600150 preps
Price: 406.00
Your Price:
-
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PureLink® Total RNA Blood Kit allows purification of high-quality total RNA from 50 - 500 µl fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol.
• RNA purification from whole blood in under an hour
• Yields high-quality RNA devoid of non-nucleated erythrocytes

Simple RNA Purification from Whole Blood
PureLink® Total RNA Blood Kit is designed for purifying from 50 to 500 µl fresh whole blood or approximately 3.5 x 106 leukocytes (white blood cells) in less than 1 hour. The entire protocol can be done in spin tubes and spin columns.

Leukocyte Enrichment for Improved RNA Purification
Whole blood consists of non-nucleated erythrocytes (red blood cells, 4 - 6 million/mm3) and leukocytes, with the latter present in the minority (5000 - 7000/mm3). Purifying total RNA from whole blood without the removal of erythrocytes results in low RNA yields and clogging of purification columns. The distinct feature of the PureLink® Total RNA Blood Kit is that it employs a leukocyte enrichment step. Hypotonic shock is used to selectively lyse erythrocytes while not affecting leukocytes. Non-nucleated erythrocytes are efficiently depleted, thus significantly reducing cell debris that may otherwise interfere with RNA purification and other downstream applications. Consequently, yields of high-quality total RNA are obtained using the PureLink® Total RNA Blood Kit.

Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:
Northern Blotting Kits & Reagents
Nuclease protection assays
Microarray
• cDNA synthesis
Real-Time PCR (qPCR)

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Specifications

For Use With (Application)
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction
No. of Reactions
50 Preps
Quantity
50
Format
Spin Column
Yield
1000 µg
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Shipping Condition
Room Temperature
Starting Material Amount
Up to 3.5 x 10^6 leukocytes, 50 to 500 µl blood
System Type
PureLink™
Product Line
PureLink™
Final Product Type
Total RNA
Purification Target
Total RNA
Sample Type
Blood
Purification Time
20 min
Isolation Technology
Silica Spin Column
Scale
Mini

Contents & Storage

Store at room temperature.