Thermo Scientific™

RevertAid First Strand cDNA Synthesis Kit

Catalog number: K1622
Thermo Scientific™

RevertAid First Strand cDNA Synthesis Kit

Catalog number: K1622
Catalog Number
K1622
Unit Size
100 reactions
Price (USD)
Price: 415.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
20 reactions
Price (USD)
Price: 102.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K1622100 reactions
Price: 415.00
Your Price:
-
K162120 reactions
Price: 102.00
Your Price:
-
Product Overview
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Additional Information
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Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), a recombinant M-MuLV RT which maintains activity at 42-50°C and is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation.
The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of the poly(A) tail, therefore, they can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis of RNA species lacking a poly(A) tail (e.g., microRNAs). Gene-specific primers may also be used with the kit.

Highlights

• Full-length first strand cDNA up to 13 kb
• Optimum reaction temperature 42°C
• Complete kit—all the components for the RT reaction are included

Applications

• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis

Specifications

For Use With (Application)
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)
Optimal Reaction Temperature
42° C
Product Line
RevertAid
Product Type
First Strand CDNA Synthesis Kit
Reverse Transcriptase
RevertAid
Sufficient For
100 reactions
No. of Reactions
100 Reactions
Reaction Format
Separate Components
Sample Type
RNA

Contents & Storage

The RevertAid First Strand cDNA Synthesis Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Oligo(dT)18 Primer, Random Hexamer Primer, Control GAPDH RNA, 10 μM Forward GAPDH Primer, 10 μM Reverse GAPDH Primer, and nuclease-free water.
Store at -20°C.