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Thermo Scientific™

GeneBLAzer™ TRα-UAS-bla HEK 293T Assay Kit

Catalog number: K1684
Thermo Scientific™

GeneBLAzer™ TRα-UAS-bla HEK 293T Assay Kit

Catalog number: K1684
Catalog Number
K1684
Unit Size
1 plate
Price (USD)
Price: 8,610.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K16841 plate
Price: 8,610.00
Your Price:
-
Product Overview
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TR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Thyroid hormone receptor alpha(TR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla HEK 293T cell line. GeneBLAzer®UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-TR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. TR alpha-UASbla HEK 293T 293 cells are functionally validated for Z' and EC50 concentrations of T3 Thryoid hormone. In addition, TR alpha-UAS-bla HEK 293T 293 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Specifications

Alternate Agonists
9-cis-retinoic acid, TTNPB, CD437, AM 580
Antagonists
AGN 193109
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Dividing Cells
Detection Method
Fluorescent
Primary Agonist
All-trans-retinoic Acid
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
THRA, TR alpha, NR1A1
System Type
GeneBLAzer™
Druggable Target
Nuclear Receptors

Contents & Storage

Shipping: Dry IceStorage: -80°C/Liquid NitrogenContains: TR alpha-UAS-bla HEK 293T cells (K1260)