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Thermo Scientific™

GeneBLAzer™ AVPR1a-NFAT-bla CHO-K1 Cells

Catalog number: K1715
Thermo Scientific™

GeneBLAzer™ AVPR1a-NFAT-bla CHO-K1 Cells

Catalog number: K1715
Catalog Number
K1715
Unit Size
2 × 10^6 cells
Price (USD)
Price: 8,690.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K17152 × 10^6 cells
Price: 8,690.00
Your Price:
-
Product Overview
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GeneBLAzer® AVPR1a-NFAT-bla CHO-K1 cells contain the human Arginine Vasopressin Receptor 1a (AVPR1a) stably integrated into the CellSensor® NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 cells (Cat. no. K1534) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element.

The GeneBLAzer® AVPR1a-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of Vasopressin. In addition, GeneBLAzer® AVPR1a-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

The family of receptors that recognize vasopressin and oxytocin consists of four members: vasopressin receptors 1a (AVPR1a), 1b (AVPR1b), and 2 (AVPR2), and the oxytocin (OT) receptor. AVPR1a, AVPR1b, and the OT receptor couple to the Gq/G11 class of G proteins and trigger the release of intracellular calcium when stimulated. AVPR2 receptors couple to Gs proteins and activate adenyl cyclase. All three AVPR receptors have high affinity for their endogenous ligand, vasopressin, but AVPR1b has less affinity for oxytocin than does AVPR1a or AVPR2. The hormones vasopressin and oxytocin are synthesized in the hypothalamus. Oxytocin causes contractions in the uterus during childbirth. The main functions of arginine vasopressin (AVP) are reabsorption of water by the kidneys and contraction of smooth muscle in arteries. This system may also play a role in memory and learning.

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Specifications

Accession Number
NM_000706.3
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Dividing Cells
Detection Method
Fluorescent
For Use With (Application)
FRET
Gene Symbol
AVPR1a
Population Status
Single Cell Clone Origin
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
AVPR1A
Cell Line
CHO-K1
System Type
GeneBLAzer™
Druggable Target
GPCRs

Contents & Storage

Contents:
K1715 GeneBLAzer™ AVPR1a-NFAT-bla CHO-K1 cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer™ AVPR1a-NFAT-bla CHO-K1 cells
• GeneBLAzer™ AVPR1a-NFAT-bla CHO-K1 Assay Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer™ AVPR1a-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.