Thermo Scientific™

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 4 Peptide

Catalog number: PV3177
Thermo Scientific™

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 4 Peptide

Catalog number: PV3177
Catalog Number
PV3177
Unit Size
800 x 20 µL assays
Price (USD)
Price: 688.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV3177800 x 20 µL assays
Price: 688.00
Your Price:
-
Product Overview
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FAQ
Citations & References
Additional Information
Recommendations
Recommendations
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase, containing Ser-7 and a consensus seq Hyd-X-R-X-X-S⁄T-X-X, where Hyd is bulky hydrophobic, F > L > V>>A, and X is any residue.

Specifications

Assay
Serine⁄Threonine Kinase Assay
Assay Entry
Biochemical phospho-specific activity (no antibody)
Excitation/Emission
405⁄465, 535
Label or Dye
Methylcoumarin
No. of Reactions
800 Reactions
Readout
End Point
References
Biochem J. vol 296, pp. 843-9 (1993), J. Biol Chem, vol. 260, pp 14471-6 (1985)
Shipping Condition
Dry Ice
Substrate Properties
Peptide-Based Substrate
Target Entry
MAPK8, MAPKAPK2, MAPKAPK5, CAMK2D, MAPK9, STK22B, STK22D, CAMK2A, MAPK14, MAPK10, MAPKAPK3
Target Enzyme
Serine⁄Threonine Kinases
Technique
FRET
Validated Kinases
MAPKAPK2, MAPKAPK3, MAPKAPK5, STK22B, STK22D, CAMK2A, CAMK2D, MAPK8, MAPK9, MAPK10, MAPK14
Product Line
Z´-LYTE™
Source
based on Ser 7 glycogen synthase (KKLNRTLSVA)
Quantity
800 x 20 μL
Format
384-well plate

Contents & Storage

Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.