As part of the LanthaScreen® TR-FRET toolbox of assay reagents, LanthaScreen® Thiol Reactive Tb Chelate labeling reagent is available for assay development. The thiol-reactive maleimide group can be conjugated to virtually any peptide or protein that contains one or more accessible thiol moieties, such as the thiol group found on a cysteine residue. Energy transfer from the terbium donor to a suitable acceptor such as fluorescein is readily detected by monitoring an increase in acceptor fluorescence intensity. See the user guide for an application-based protocol for cysteine-containing peptides or proteins.
Contents & Storage
LanthaScreen™ TR-FRET Labeling Reagents are provided lyophilized. Store at -20°C, desiccated, and protected from light. After reconstitution, terbium lanthanide chelates should be stored on ice and used within the same day.