Informational:This kit contains a goat Tb-anti-GST antibody—an equivalent kit containing a rabbit-sourced antibody is also available.

LanthaScreen™ TR-FRET RAR alpha Coactivator Assay Kit, goat

Catalog number: PV4409

LanthaScreen™ TR-FRET RAR alpha Coactivator Assay Kit, goat

Catalog number: PV4409
Catalog Number
PV4409
Unit Size
800 x 20 µL assays
Price (USD)
Price: 1,752.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV4409800 x 20 µL assays
Price: 1,752.00
Your Price:
-
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This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15122:

The LanthaScreen® TR-FRET Retinoic Acid Receptor (RAR) alpha Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential RAR alpha ligands as agonists of coactivator recruitment. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

How it works

The LanthaScreen® TR-FRET RAR alpha Coactivator Assay Kit includes a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human RAR alpha ligand-binding domain (RAR alpha-LBD) that is tagged with glutathione-S-transferase (GST). To assay, RAR alpha-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti-GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound.

Contents and Storage:

The LanthaScreen® TR-FRET RAR alpha Coactivator Assay Kit contains RAR alpha-LBD (GST) protein, fluorescein-labeled D22 coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

Specifications

Format
384-well plate
Product Line
LanthaScreen™
Technique
TR-FRET
Detection Method
Fluorescent
For Use With (Equipment)
Microplate Reader
Assay Entry
Biochemical coregulator interaction
Gene ID (Entrez)
5914
Readout
End Point
Shipping Condition
Dry Ice
Target Entry
RARA, RAR alpha, NR1B1
Validated Application
Co-Factor Interaction Assay
Label or Dye
Tb (Terbium)

Contents & Storage

Store in ultra-cold freezer (-68 to -85°C).