Informational:This kit contains a goat Tb-anti-GST antibody—an equivalent kit containing a rabbit-sourced antibody is also available.
Thermo Scientific™

LanthaScreen™ TR-FRET VDR Coactivator Assay Kit, goat

Catalog number: PV4554
Thermo Scientific™

LanthaScreen™ TR-FRET VDR Coactivator Assay Kit, goat

Catalog number: PV4554
Catalog Number
PV4554
Unit Size
800 x 20 µL assays
Price (USD)
Price: 1,782.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV4554800 x 20 µL assays
Price: 1,782.00
Your Price:
-
Product Overview
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This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15127:

The LanthaScreen® TR-FRET Vitamin D Receptor (VDR) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential VDR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a VDR ligand-binding domain (VDR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format.

The assay
To run the LanthaScreen® TR-FRET VDR Coactivator Assay, VDR-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti-GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based upon the biology of the VDRcoactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

Contents and Storage:
The LanthaScreen® TR-FRET VDR Coactivator Assay Kit contains the VDR-LBD (GST) protein, fluorescein-labeled TRAP220/DRIP-2 coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

Specifications

Format
384-well plate
Product Line
LanthaScreen™
Technique
TR-FRET
Detection Method
Fluorescent
For Use With (Equipment)
Microplate Reader
Quantity
800 x 20 μL Assays
Assay Entry
Biochemical coregulator interaction
Gene ID (Entrez)
7421
Readout
End Point
Shipping Condition
Dry Ice
Target Entry
VDR, NR1I1
Validated Application
Co-Factor Interaction Assay
Label or Dye
Tb (Terbium)

Contents & Storage

Store in ultra-cold freezer (-68 to -85°C).