Informational:This kit contains a goat Tb-anti-GST antibody—an equivalent kit containing a rabbit-sourced antibody is also available.

LanthaScreen™ TR-FRET PPAR delta Coactivator Assay Kit, goat

Catalog number: PV4685

LanthaScreen™ TR-FRET PPAR delta Coactivator Assay Kit, goat

Catalog number: PV4685
Catalog Number
PV4685
Unit Size
800 x 20 µL assays
Price (USD)
Price: 1,738.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV4685800 x 20 µL assays
Price: 1,738.00
Your Price:
-
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Recommendations
This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15134:

The LanthaScreen® TR-FRET PPAR (peroxisome proliferator activated receptor) delta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential PPAR delta ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant PPAR delta ligand-binding domain (PPAR delta-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.

When running the LanthaScreen® TR-FRET PPAR delta Coactivator Assay in agonist mode (to identify agonist compounds), PPAR delta-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-labeled coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the TR-FRET ratio of 520/495 is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the PPAR delta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (see Figure 1).

Contents and Storage:
The LanthaScreen® TR-FRET PPAR delta Coactivator Assay Kit contains PPAR delta-LBD (GST) protein, fluorescently labeled C33 coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C or 4°C).

Specifications

Format
384-well plate
Product Line
LanthaScreen™
Technique
TR-FRET
Detection Method
Fluorescent
For Use With (Equipment)
Microplate Reader
Assay Entry
Biochemical coregulator interaction
Gene ID (Entrez)
5467
Readout
End Point
Shipping Condition
Dry Ice
Target Entry
PPAR delta, NR1C2, PPARD
Validated Application
Co-Factor Interaction Assay
Label or Dye
Tb (Terbium)

Contents & Storage

Store in ultra-cold freezer (-68 to -85°C).