Informational:This kit contains a goat Tb-anti-GST antibody—an equivalent kit containing a rabbit-sourced antibody is also available.
Thermo Scientific™

LanthaScreen™ TR-FRET TR beta Coactivator Assay Kit, goat

Catalog number: PV4686
Thermo Scientific™

LanthaScreen™ TR-FRET TR beta Coactivator Assay Kit, goat

Catalog number: PV4686
Catalog Number
PV4686
Unit Size
800 x 20 µL assays
Price (USD)
Price: 1,754.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV4686800 x 20 µL assays
Price: 1,754.00
Your Price:
-
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This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15135:

The LanthaScreen® TR-FRET TR (thyroid receptor) beta Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential TR beta ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant TR beta ligand-binding domain (TR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous, mix-and-read assay format.

To Assay:
When performing the LanthaScreen® TR-FRET TR beta coactivator assay, TR beta-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the TR beta-coactivator peptide interaction, the ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, induce a conformational change, and recruit coactivator peptide (Figure 1).

Contents and Storage:
The LanthaScreen® TR-FRET TR beta Coactivator Assay Kit contains TR beta-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

Specifications

Format
384-well plate
Product Line
LanthaScreen™
Technique
TR-FRET
Detection Method
Fluorescent
For Use With (Equipment)
Microplate Reader
Assay Entry
Biochemical coregulator interaction
Readout
End Point
Shipping Condition
Dry Ice
Target Entry
T3RB, TR beta, THRB, NR1A2
Validated Application
Co-Factor Interaction Assay
Label or Dye
Tb (Terbium)

Contents & Storage

Store in ultra-cold freezer (-68 to -85°C).