Informational:This kit contains a goat Tb-anti-GST antibody—an equivalent kit containing a rabbit-sourced antibody is also available.
Thermo Scientific™

LanthaScreen™ TR-FRET TR alpha Coactivator Assay Kit, goat

Catalog number: PV4687
Thermo Scientific™

LanthaScreen™ TR-FRET TR alpha Coactivator Assay Kit, goat

Catalog number: PV4687
Catalog Number
Unit Size
800 x 20 µL assays
Price (USD)
Price: 1,756.00
Your Price:
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV4687800 x 20 µL assays
Price: 1,756.00
Your Price:
Product Overview
Citations & References
Additional Information
This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15136:

The LanthaScreen® TR-FRET TR (thyroid receptor) alpha Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential TR alpha ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant TR alpha ligand-binding domain (TR alpha-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous, mix-and-read assay format.

To Assay:
When performing the LanthaScreen® TR-FRET TR alpha coactivator assay, TR alpha-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based on the biology of the TR alpha-coactivator peptide interaction, the ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, induce a conformational change, and recruit coactivator peptide (Figure 1).

Contents and Storage:
The LanthaScreen® TR-FRET TR alpha Coactivator Assay Kit contains TR alpha-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).


384-well plate
Product Line
Detection Method
For Use With (Equipment)
Microplate Reader
Assay Entry
Biochemical coregulator interaction
End Point
Shipping Condition
Dry Ice
Target Entry
THRA, TR alpha, NR1A1
Validated Application
Co-Factor Interaction Assay
Label or Dye
Tb (Terbium)

Contents & Storage

Store in ultra-cold freezer (-68 to -85°C).