Informational:This kit contains a goat Tb-anti-GST antibody—an equivalent kit containing a rabbit-sourced antibody is also available.
Thermo Scientific™

LanthaScreen™ TR-FRET ERR beta Coactivator Assay Kit, goat

Catalog number: PV4800
Thermo Scientific™

LanthaScreen™ TR-FRET ERR beta Coactivator Assay Kit, goat

Catalog number: PV4800
Catalog Number
PV4800
Unit Size
800 x 20 µL assays
Price (USD)
Price: 1,814.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV4800800 x 20 µL assays
Price: 1,814.00
Your Price:
-
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This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15139:

The LanthaScreen® TR-FRET ERR (estrogen related receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential ERR beta ligands as inverse agonists of ligand-dependent coactivator displacement. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant ERR beta ligand-binding domain (ERR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.

To assay:

When running the LanthaScreen® ERR beta TR-FRET Coactivator Assay, ERR beta-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound.
Based on the biology of the ERR beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide (see Figure 1).

Specifications

Format
384-well plate
Product Line
LanthaScreen™
Technique
TR-FRET
Detection Method
Fluorescent
For Use With (Equipment)
Microplate Reader
Assay Entry
Biochemical coregulator interaction
Readout
End Point
Shipping Condition
Dry Ice
Target Entry
NR3B2, ERR2, ESRRB
Validated Application
Co-Factor Interaction Assay
Label or Dye
Tb (Terbium)

Contents & Storage

The LanthaScreen™ TR-FRET ERR beta Coactivator Assay Kit contains ERR beta-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-labeled anti-GST antibody, and buffers.

Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).