Informational:This kit contains a goat Tb-anti-GST antibody—an equivalent kit containing a rabbit-sourced antibody is also available.
Thermo Scientific™

LanthaScreen™ TR-FRET CAR Coactivator Assay Kit, goat

Catalog number: PV4836
Thermo Scientific™

LanthaScreen™ TR-FRET CAR Coactivator Assay Kit, goat

Catalog number: PV4836
Catalog Number
PV4836
Unit Size
800 x 20 µL assays
Price (USD)
Price: 1,802.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV4836800 x 20 µL assays
Price: 1,802.00
Your Price:
-
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Recommendations
This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15141:

The LanthaScreen® TR-FRET Constitutive Androstane Receptor (CAR) Coactivator Assay provides a sensitive and robust method for highthroughput screening (HTS) of potential CAR ligands as agonists of ligand-dependent coactivator recruitment or inverse agonists of liganddependent coactivator displacement. The kit uses a terbium-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a CAR ligand-binding domain (CAR-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.

To assay:
When running the LanthaScreen® TR-FRET CAR Coactivator Assay, CAR-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and terbium anti-GST antibody. After an incubation period at room temperature, the 520 nm/ 495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the CAR-coactivator peptide interaction, this ligand’s EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and either recruit or displace coactivator peptide (Figures 1 and 2).

Specifications

Format
384-well plate
Product Line
LanthaScreen™
Technique
TR-FRET
Detection Method
Fluorescent
For Use With (Equipment)
Microplate Reader
Assay Entry
Biochemical coregulator interaction
Readout
End Point
Shipping Condition
Dry Ice
Target Entry
CAR, NR1I3
Validated Application
Co-Factor Interaction Assay
Label or Dye
Tb (Terbium)

Contents & Storage

The LanthaScreen™ TR-FRET CAR Coactivator Assay Kit contains CAR-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, terbium-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).