Informational:This kit contains a goat Tb-anti-GST antibody—an equivalent kit containing a rabbit-sourced antibody is also available.

LanthaScreen™ TR-FRET PPAR delta Competitive Binding Assay Kit, goat

Catalog number: PV4893

LanthaScreen™ TR-FRET PPAR delta Competitive Binding Assay Kit, goat

Catalog number: PV4893
Catalog Number
PV4893
Unit Size
400 x 40 µL
Price (USD)
Price: 1,726.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV4893400 x 40 µL
Price: 1,726.00
Your Price:
-
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This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15144:

The LanthaScreen® TR-FRET PPAR delta Competitive Binding Assay provides a sensitive and robust method for high-throughput screening (HTS) of ligands for peroxisome proliferator-activated receptor delta (PPAR delta). The kit uses a terbium-labeled anti-GST antibody, a fluorescent small-molecule pan-PPAR ligand (Fluormone™ Pan-PPAR Green), and a human PPAR delta ligand-binding domain (LBD) that is tagged with glutathione S-transferase (GST), in a homogeneous mix-and-read assay format.

To assay:
When running the LanthaScreen® TR-FRET PPAR delta Competitive Binding Assay, Fluormone™ Pan-PPAR Green is added to ligand test compounds, followed by addition of a mixture of the PPAR delta-LBD and terbium anti-GST antibody. When the Fluormone™ Pan-PPAR Green is bound to PPAR delta, energy transfer from the terbium-labeled antibody to the tracer occurs, and a high TR-FRET ratio is observed. Competitive ligand binding to PPAR delta is detected by a test compound’s ability to displace the tracer, which results in a loss of FRET between the antibody and the tracer. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the IC50 from a dose response curve of the compound (Figure 1). This type of binding assay is analogous to radioligandbased assays, except that it eliminates handling of radioactivity and enables a homogeneous, "addition-only" format.

Specifications

Format
384-well plate
Product Line
LanthaScreen™
Technique
TR-FRET
Detection Method
Fluorescent
For Use With (Equipment)
Microplate Reader
Assay Entry
Biochemical competitive binding
Readout
End Point
Shipping Condition
Dry Ice
Target Entry
PPAR delta, NR1C2, PPARD
Validated Application
Competitive Binding Assay
Label or Dye
Tb (Terbium)

Contents & Storage

The LanthaScreen™ TR-FRET PPAR delta Competitive Binding Assay Kit contains PPAR delta-LBD (GST) protein, Fluormone™ Pan-PPAR Green, terbium-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).