Kinase Tracer 199, 25 µM

Catalog number: PV5830

Kinase Tracer 199, 25 µM

Catalog number: PV5830
Catalog Number
PV5830
Unit Size
25 µL
Price (USD)
Price: 476.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV583025 µL
Price: 476.00
Your Price:
-
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Recommendations
In the LanthaScreen® Eu Kinase Binding Assay, binding of an Alexa Fluor® conjugate or 'tracer' such as Kinase Tracer 199 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps. Visit www.invitrogen.com/bindingassay to learn more and to see which kinases have been validated using this kinase tracer.

Specifications

Assay
Kinase Binding Assay
Assay Entry
Biochemical binding/kinetic
Color
Far-Red
Detection Method
Fluorescent
Excitation/Emission
340/665
For Use With (Application)
TR-FRET
Label or Dye
Alexa Fluor™ 647
Shipping Condition
Dry Ice
Target Entry
MAPK8, EGFR, RIPK2, MAPK9, EGFR T790M L858R, MAPK14, MAPK10, EGFR L861Q, EGFR L858R
Validated Kinases
EGFR T790M L858R, EPHB4, KIT V654A, MAPK9 (JNK2), RIPK2

Contents & Storage

Store at -20°C.