Informational:This PolarScreen™ PPARγ-Competitor Assay Kit, Green replaces a previous version of the kit (catalog number PV3355). Both versions perform similarly. The new kit contains a GST-tagged PPARγ-LBD while the old kit was His-tagged.

PolarScreen™ PPARγ-Competitor Assay Kit, Green

Catalog number: PV6136

PolarScreen™ PPARγ-Competitor Assay Kit, Green

Catalog number: PV6136
Catalog Number
PV6136
Unit Size
800 x 20 µL
Price (USD)
Price: 1,630.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV6136800 x 20 µL
Price: 1,630.00
Your Price:
-
Product Overview
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The PPARγ (Peroxisome Proliferator-Activated Receptor-gamma) Competitor Assay, Green uses the human-derived recombinant PPARγ ligand-binding domain (PPARγ-LBD) tagged with an N-terminal GST-tag and a novel, tight-binding, selective fluorescent PPARγ ligand (Fluormone™ PPARγ Green) in a homogenous, mix-and-read assay format. PPARγ-LBD/Fluormone™ PPARγ Green complex produces high polarization. This complex is then added to individual test compounds in microwell plates. Competitors displace the fluorescent Fluormone™ PPARγ Green Ligand from the PPARγ-LBD/Fluormone™ PPARγ Green complex, causing the fluorescent ligand to tumble rapidly during its fluorescence lifetime, resulting in a low polarization value. Noncompetitors will not displace the fluorescent ligand from the complex, so the polarization value remains high. The shift in polarization value in the presence of the test compounds is used to determine relative affinity of test compounds for the PPARγ-LBD.
WARNING: Reproductive Harm - www.P65Warnings.ca.gov

Specifications

Format
384-well plate
Product Line
PolarScreen™
Technique
Fluorescence Polarization
Color
Green
Detection Method
Fluorescent
For Use With (Equipment)
Microplate Reader
Assay Entry
Biochemical competitive binding
Excitation/Emission
485⁄535
Gene ID (Entrez)
5468
Readout
End Point
Shipping Condition
Dry Ice
Target Entry
PPARG, PPAR gamma, NR1C3
Validated Application
Competitive Binding Assay

Contents & Storage

PV3356 — Fluormone™ PPARγ Green (-20°C)
PV4546 — PPARγ-LBD (-80°C)
PV3358 — PPARγ Green Screening Buffer (room temperature)
P2325 — 1M DTT (-80°C or -20°C)