Thermo Scientific™

ULK1 Recombinant Human Protein

Catalog number: PV6432
Thermo Scientific™

ULK1 Recombinant Human Protein

Catalog number: PV6432
Catalog Number
PV6432
Unit Size
1 mg
Price (USD)
Price: 7,380.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
100 µg
Price (USD)
Price: 1,650.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV64321 mg
Price: 7,380.00
Your Price:
-
PV6431100 µg
Price: 1,650.00
Your Price:
-
Product Overview
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Additional Information
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ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner. Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay

Specifications

Molecular Weight
96.1 kDa
Quantity
1 mg
Gene Alias
ATG1, UNC51, Unc51.1, FLJ38455
Protein Length
aa 1-649
Recombinant
Yes
Protein Tag
GST Tag
Kinase Group
Serine/Threonine Kinase
Expression System
Baculovirus
Protein Form
Recombinant
Form
Liquid
Validated Application
Kinase Assay
Species
Human

Contents & Storage

Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.