Selectively isolate Salmonellae, other than Salmonella enterica serovars Typhi and Paratyphi from food samples or other material using Thermo Scientific™ Remel™ Brilliant Green Agar (Dehydrated). Kristensen developed Brilliant Green Agar for isolation of Salmonella1. Moats added novobiocin to the medium which inhibited growth of Proteus in cultures from beef and poultry products2. Brilliant Green Agar is recommended by the American Public Health Association (APHA) for selective isolation of Salmonella from food products3.
|Description||Brilliant Green Agar|
|Certifications/Compliance||Industrial Reference: SMWW, USDA, USP.|
|Catalog Number||Specifications||Unit Size||Quantity||Price (USD)|
|R452552||Each||500 g||Request A Quote|
This medium contains casein and meat peptones which provide nitrogen, amino acids and peptides necessary for bacterial growth. Yeast extract is a source of B-complex vitamins. Sodium chloride is a source of essential electrolytes and maintains osmotic equilibrium. Lactose- and sucrose-fermenting colonies occasionally growing on this medium will be yellow-green in color surrounded by a yellow-green zone. Salmonella colonies range from reddish or pink to nearly white in color with a red zone. Novobiocin suppresses the growth of Proteus and, to a lesser extent, Escherichia coli, Citrobacter, and Pseudomonas. In separate studies, Devenish and Cooke added novobiocin to Brilliant Green Agar to further improve selectivity4,5.
Easy-to-read – Phenol red in the medium serves as an acid base indicator giving yellow color to lactose and sucrose fermenting bacteria.