Micro electron diffraction (MicroED) allows fast, high-resolution 3D structure determination of small chemical compounds and biological macromolecules. Thermo Fisher Scientific now offers a complete MicroED solution that is compatible with new microscopes and can be retrofitted onto existing units.
MicroED data is acquired on a cryo-transmission electron microscope (cryo-TEM), using electrons as the incident beam. Since it is a diffraction technique, like X-ray crystallography, the sample needs to be crystalline.
The crystallization process is essentially the same as for X-ray crystallography. However, much smaller crystals can be used because the interaction of the crystal with electrons is much stronger than it is with x-rays. Crystals well below 100 nm in size can readily be analyzed. This may significantly shorten the sample preparation process and allows for the analysis of crystals, which are too small to diffract with other methods.
Data collection is completed in only a few minutes, and 3D structures can be determined at atomic resolution.
With the addition of our complete MicroED solution, your Thermo Scientific cryo-TEM becomes your own in-house beam line for structure determination!
- Fast atomic-resolution 3D structural information. Diffraction data from nanocrystals in minutes.
- Instant productivity. Nanocrystals as small as 100 nm can be readily analyzed, removing the burden of growing large crystals (as with X-ray crystallography). Also reduces the amount of sample material required. Mixtures of different polymorphs and compounds can be analyzed.
- Complete turnkey solution. Including hardware, software, and support from one single vendor. Acquired data can be readily processed using established reconstruction packages for X-ray crystallography.
- 2-in-1 solution. MicroED and single particle analysis (SPA) can be performed on the same cryo-electron microscope. This solution is compatible with new microscopes but is also retrofittable on existing units.