Electron Microscopy

MicroED – Microcrystal Electron Diffraction

Cryo-EM diffraction method for structure determination of small chemical compounds and biological macromolecules.


Micro electron diffraction (MicroED) allows fast, high-resolution 3D structure determination of small chemical compounds and biological macromolecules. Thermo Fisher Scientific now offers a complete MicroED solution that is compatible with new microscopes and can be retrofitted onto existing units.

MicroED data is acquired on a cryo-transmission electron microscope (cryo-TEM), using electrons as the incident beam. Since it is a diffraction technique, like X-ray crystallography, the sample needs to be crystalline.

The crystallization process is essentially the same as for X-ray crystallography. However, much smaller crystals can be used because the interaction of the crystal with electrons is much stronger than it is with x-rays. Crystals well below 100 nm in size can readily be analyzed. This may significantly shorten the sample preparation process and allows for the analysis of crystals, which are too small to diffract with other methods.

Data collection is completed in only a few minutes, and 3D structures can be determined at atomic resolution.

With the addition of our complete MicroED solution, your Thermo Scientific cryo-TEM becomes your own in-house beam line for structure determination!

Proteinase structure determined with microcrystal electron diffraction.
Proteinase K structure. MicroED data provided by the Gonen Laboratory HHMI/UCLA.

Key Benefits

  • Fast atomic-resolution 3D structural information. Diffraction data from nanocrystals in minutes.
  • Instant productivity. Nanocrystals as small as 100 nm can be readily analyzed, removing the burden of growing large crystals (as with X-ray crystallography). Also reduces the amount of sample material required. Mixtures of different polymorphs and compounds can be analyzed.
  • Complete turnkey solution. Including hardware, software, and support from one single vendor. Acquired data can be readily processed using established reconstruction packages for X-ray crystallography.
  • 2-in-1 solution. MicroED and single particle analysis (SPA) can be performed on the same cryo-electron microscope. This solution is compatible with new microscopes but is also retrofittable on existing units.

Applications

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Structural Biology Research
 

Cryo-electron microscopy enables the structural analysis of challenging biological targets such as large complexes, flexible species and membrane protein.

DrugDiscovery_Appl_274x180

Drug Discovery
 

Learn how to take advantage of rational drug design for many more major drug target classes, leading to best-in-class drugs.


Samples


Proteins Analysis

Cryo-electron microscopy provides near-atomic resolution 3D protein structure. It can determine structural information for complexes and crystallization-resistant samples, as well as vital cellular context.

Learn more ›


Biopharmaceutical Research

Structural drug discovery is enabled by cryo-electron microscopy, as the method provides near-atomic-resolution detail for small molecules and protein biologics in their fully hydrated state.

Learn more ›


Products

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Krios Rx Cryo-TEM

  • Industry leading productivity and ease of use
  • Fixed 300 kV voltage for SPA only
  • Pharma-dedicated service package for guaranteed reliability

Krios G4 Cryo-TEM

  • Improved ergonomics
  • Fits more easily into new and existing labs
  • Maximized productivity and automation
  • Best image quality for high-resolution 3D reconstruction

Glacios Cryo-TEM

  • Flexible Accelerating Voltage 80-200 kV
  • Industry-leading Autoloader for cryogenic sample manipulation
  • Small footprint
  • Enhanced ease-of-use

Vitrobot System

  • Fully Automated Sample vitrification
  • Blotting Device
  • Semi-Automated Grid Transfer
  • High Sample Throughput

Talos Arctica TEM

  • Increased data acquisition speed
  • High data with robotic sample handling & automated loading
  • Unattended platform operation and automated data acquisition
  • Low cost of ownership with remote diagnostics and preventive service

Talos F200C TEM

  • Flexible EDS analysis reveals chemical information
  • High-contrast, high-quality TEM and STEM imaging
  • Ceta 16 Mpixel CMOS camera provides large field of view and high read-out speed

Ceta-D Camera

  • Optimum performance at any high tension (20–300 kV)
  • Compatible with post-column filters and spectrometers
  • Movie acquisition for dynamic studies
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