Thermo Fisher Scientific Logo
      • Popular
      • Applications & Techniques
      • Shop All Products
      • Services
      • Support
      • Order Status
      • Sign in
      • Quick Order
      •    

      Search Thermo Fisher Scientific

      Search All
      search clear

          Benchmarking the Q Exactive HF-X MS for Shotgun Proteomics

          • Overview
          • Presenter

          Key learning objectives of this seminar:

          • Methods for sample preparation and LC and MS methods for high-throughput shotgun proteomics
          • MS acquisition methodology used for very deep single-shot proteomics
          • Multiplexed tandem mass tag quantification methods on the Thermo Scientific Q Exactive HF-X Hybrid Quadrupole-Orbitrap mass spectrometer for TMT 10-plex

          With advances in mass resolution, mass accuracy, fragmentation technology, and speed, bottom-up analysis can identify more proteins in more-complex samples than ever. Moreover, the techniques and principles involved are generally foundational for more complex experiments. Here, we evaluate the performance of the newly introduced Q Exactive HF-X MS. Various MS methods and LC gradient lengths were evaluated for bottom-up proteomics. The largest gains were found at ultrashort gradients with a fast acquisition method resulting in more than 1,100 unique peptide sequences per minute. Additional data will be shown for phosphoproteomics with tandem isobaric mass tag (TMT10-plex) and data-independent acquisition (DIA) analysis on the new Q Exactive HF-X mass spectrometer.

          Sign up to watch the webinar and download the executive summary detailing:

          • The hardware and software improvements that enable the Q Exactive HF-X MS to push the speed and sensitivity limits of protein identification
          gen-exec-summary-screenshot-02-450x450px

          About the presenter

          Jesper Olsen, Ph.D, Full Professor, Novo Nordisk Foundation Center for Protein Research (CPR), University of Copenhagen

          Jesper Olsen, Ph.D, Full Professor
          Novo Nordisk Foundation Center for Protein Research (CPR)
          University of Copenhagen

          Jesper Olsen studied analytical chemistry at the University of Southern Denmark in Odense, and obtained his PhD in biochemistry and molecular biology in the laboratory of Matthias Mann. During his PhD he was involved in developing high-resolution mass spectrometry-based proteomics. He spent 4 years as a post-doctoral fellow at the Max Planck Institute for Biochemistry in Munich, where he developed a quantitative phosphoproteomics technology that was applied to global time-resolved analyses of cell signaling pathways in human cells.

          In 2009, Jesper was recruited back to Denmark to head a group at the newly established Novo Nordisk Foundation Center for Protein Research (CPR) at University of Copenhagen. In 2012, he was promoted to vice director of CPR and in 2014 full professor. Jesper has received a number of research awards including the Max Planck Institute for Biochemistry Junior Research Award and HUPO Young Investigator Award in Proteomic Sciences.

          Overview

          Key learning objectives of this seminar:

          • Methods for sample preparation and LC and MS methods for high-throughput shotgun proteomics
          • MS acquisition methodology used for very deep single-shot proteomics
          • Multiplexed tandem mass tag quantification methods on the Thermo Scientific Q Exactive HF-X Hybrid Quadrupole-Orbitrap mass spectrometer for TMT 10-plex

          With advances in mass resolution, mass accuracy, fragmentation technology, and speed, bottom-up analysis can identify more proteins in more-complex samples than ever. Moreover, the techniques and principles involved are generally foundational for more complex experiments. Here, we evaluate the performance of the newly introduced Q Exactive HF-X MS. Various MS methods and LC gradient lengths were evaluated for bottom-up proteomics. The largest gains were found at ultrashort gradients with a fast acquisition method resulting in more than 1,100 unique peptide sequences per minute. Additional data will be shown for phosphoproteomics with tandem isobaric mass tag (TMT10-plex) and data-independent acquisition (DIA) analysis on the new Q Exactive HF-X mass spectrometer.

          Sign up to watch the webinar and download the executive summary detailing:

          • The hardware and software improvements that enable the Q Exactive HF-X MS to push the speed and sensitivity limits of protein identification
          gen-exec-summary-screenshot-02-450x450px
          Presenter

          About the presenter

          Jesper Olsen, Ph.D, Full Professor, Novo Nordisk Foundation Center for Protein Research (CPR), University of Copenhagen

          Jesper Olsen, Ph.D, Full Professor
          Novo Nordisk Foundation Center for Protein Research (CPR)
          University of Copenhagen

          Jesper Olsen studied analytical chemistry at the University of Southern Denmark in Odense, and obtained his PhD in biochemistry and molecular biology in the laboratory of Matthias Mann. During his PhD he was involved in developing high-resolution mass spectrometry-based proteomics. He spent 4 years as a post-doctoral fellow at the Max Planck Institute for Biochemistry in Munich, where he developed a quantitative phosphoproteomics technology that was applied to global time-resolved analyses of cell signaling pathways in human cells.

          In 2009, Jesper was recruited back to Denmark to head a group at the newly established Novo Nordisk Foundation Center for Protein Research (CPR) at University of Copenhagen. In 2012, he was promoted to vice director of CPR and in 2014 full professor. Jesper has received a number of research awards including the Max Planck Institute for Biochemistry Junior Research Award and HUPO Young Investigator Award in Proteomic Sciences.

          Fill out the form to view the free
          on-demand webinar and download the executive summary

          * Required field

          *
          *
          *
          *
          *
          *
          *
          *
          *
           I would like to speak with an expert to learn how Thermo Fisher Scientific products can help in my laboratory
           Please confirm you would like to receive marketing and promotional email messages about Thermo Fisher Scientific products and services.
           
           

          Resources

          • Orbitrap LC-MS
          • Q Exactive HF-X MS

          Literature

          • Q Exactive HF-X MS Brochure
          • The Power of Quantitative Multiplexing Poster
          • Mass Spectrometry Sample Prep Handbook

          Support

          • Unity Lab Services
          • United States Training Courses
          • European Training Courses
          •  
          •  
          •  
          •  
          •  
          •  
          •  
          Brands
          •  
          Shopping Tools
          • Product Selection Guides
          • Quick Order
          • Redeem a Quote
            * Please enter a valid quote
          • New Products
          • Promotions
          • Mobile & Desktop Apps
          • Shared Lists
          • Check Order Status
          eSolutions
          • eProcurement
          • Supply Center
          • Instrument Management
          Support
          • Check Order Status
          • Order Support
          • Training
          • Webinars
          • Blog
          • Social Media
          • Contact Us
          • Report a Site Issue
          Thermo Fisher Scientific
          • About Us About Us
          • Careers Careers
          • Investors Investors
          • News News
          • Responsibility
          • Trademarks
          • Terms & Conditions
          • Privacy Information Center
          • Price & Freight Policy

           

          Sign in

          Don't have an account ? Create Account

        • Aspire Member Program
        • Connect: Lab, Data, Apps
        • Custom Products & Projects
        • Instrument Management
        • Account
        • Check Order Status
          • Popular
              • Popular
                • TaqMan Real-Time PCR Assays TaqMan Real-Time PCR Assays
                • Antibodies Antibodies
                • Oligos, Primers & Probes Oligos, Primers & Probes
                • GeneArt Gene Synthesis GeneArt Gene Synthesis
                • Cell Culture Plastics Cell Culture Plastics
          • Applications & Techniques
            • See Links For Applications & Techniques
              • Brands
                • See Links For Brands
                • Thermo Scientific
                • Applied Biosystems
                • Invitrogen
                • Fisher Scientific
                • Unity Lab Services
                • Patheon
              • Life Sciences
                • See Links For Life Sciences
                • Antibodies
                • Cell Analysis
                • Cell Culture & Transfection
                • Cloning
                • DNA & RNA Extraction & Analysis
                • Epigenetics & ncRNA Research
                • Flow Cytometry
                • Gene Expression Analysis & Genotyping
                • Genome Editing
                • Microarray Analysis
                • Oligos, Primers, Probes & Genes
                • PCR
                • Protein Biology
                • Real-Time PCR
                • RNAi
                • Sequencing
                • Stem Cell Research
                • Synthetic Biology
              • Industrial & Applied Sciences
                • See Links For Industrial & Applied Sciences
                • Agrigenomics
                • Animal Health
                • Bioprocessing
                • Biotechnology
                • Biotech Company Solutions
                • Cement, Coal & Minerals
                • Chromatography
                • Drug Discovery & Development
                • Electron Microscopy
                • Environmental
                • Food & Beverage
                • Forensics
                • Geosciences
                • Manufacturing & Processing
                • Mass Spectrometry
                • Materials Science
                • Microbiology
                • Pharma & Biopharma
                • Radiation Detection & Measurement
                • Safety & Security Threat Detection
                • Semiconductor Analysis
                • Spectroscopy, Elemental & Isotope Analysis
              • Clinical & Diagnostics
                • See Links For Clinical & Diagnostics
                • Allergy Diagnostics
                • Anatomical Pathology
                • Autoimmune Diagnostics
                • Biobanking
                • Cancer Research
                • Cell & Gene Therapy
                • Clinical Genomics
                • Clinical Mass Spectrometry
                • Clinical Microbiology
                • Clinical & Translational Research
                • Diagnostic Development
                • Diagnostic Testing
                • Precision Medicine
                • Preclinical to Companion Diagnostic Development
                • Public Health
              • Lab Solutions
                • See Links For Lab Solutions
                • Chemicals
                • Lab Equipment
                • Lab Plasticware & Supplies
                • Sample Storage & Management
              • Digital Solutions
                • See Links For Digital Solutions
                • Lab Informatics
                • Connect Platform
                • Instrument Connectivity
                • Apps & Analysis Software
                • Lab Automation
                • Digital Services
                • Digital Commerce
          • Shop All Products
            • See Links For Shop All Products
              • Save up to 58% off cell biology products  
              • Save up to 22% on western blotting  
              • Upgrade your gel tank for $21  
              • Bundle & Save for SARS-CoV-2 research  
              • Most Popular Products
                • Pipettes & Pipette Tips
                • Beakers
                • Nalgene Labware
                • Lab Centrifuges
                • Ultra Low–Temperature Freezers
                • Lipofectamine Reagents
                • Oligos, Primers, Probes & Genes
                • SuperScript Reverse Transcriptase
                • TaqMan Real-Time PCR Assays
                • TRIzol Reagents
                • Antibodies
                • ELISA Kits
              • Most Popular Categories
                • Microplates
                • PCR Equipment and Supplies
                • Molecular Biology Reagents and Kits
                • Chromatography Columns and Cartridges
                • Chromatography Supplies
                • Cell Culture Media
                • Microbiological Media and Media Additives
                • Antibiotics and Supplements
                • Cell Analysis Products
                • Gel Electrophoresis Equipment and Supplies
                • Western Blot Products
                • Microplate Readers and Accessories
                • Spectroscopy
              • Order Tools
                • How to Order
                • Quick Order
                • Track Your Order
                • Order History
                • Order Support
                • Contact Us
              • Shop All Products
                • See Links For Shop All Products
                • Product Selection Guides
                • New Products
                • Promotions
                • Online Offers
          • Services
            • See Links For Services
                • Services Marketplace
                • Custom Services
                • Enterprise-Level Lab Informatics
                • Enterprise Services
                • Financial & Leasing Services
                • Instrument Services
                • OEM & Commercial Supply
                • Training Services
                • CDMO & Clinical Trial Services
          • Support
            • See Links For Support
              • Product Documentation
                • Manuals
                • Safety Data Sheets (SDS)
                • Certificates of Analysis & Conformance
                • CE Declarations of Conformity
                • RoHS Certificates
                • Manufacturing Site ISO Certifications
              • Other Product Information
                • Citations & References
                • Product Literature
                • Vector Maps
                • Chemical Structures
                • Spectral Data
                • Media Formulations
              • Product Support
                • Knowledge Base & FAQs
                • Instrument Support
                • Support Centers
                • Contact Us
              • Educational Resources
                • Learning Centers
                • Digital Transformation
                • Gibco Cell Culture Basics
                • Protein Methods Library
                • Supplemental Protocols
                • Newsletters & Journals
                • Training Services
                • Events
              • Popular Tools & Calculators
                • Lab Apps for Mobile Devices
                • Oligos Tools & Utilities Hub
                • Tm Calculator for PCR
                • Molecular Biology Tools
                • Gibco Media Formulation Tool
                • Cell Culture Useful Numbers
                • Fluorescence SpectraViewer
                • Flow Cytometry Panel Builder
                • Cell Staining Tool
                • Protein Gel Conversion Tool
                • Switch to Nunc Plastics Tool
                • Transfection Protocol Calculator