Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Tubes
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Clinical Genomics
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • CDMO & Clinical Trial Services
    • Custom Services
    • Enterprise Services
    • Financial & Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM & Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • Register for an Account
    • How to Order
    • Instrument Support
    • Technical Support Centers
    • Learning Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
    • Position 6
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates

Search Thermo Fisher Scientific

Search All
Search button Close
        • Order Status
        • Quick Order
        • Sign in
          Sign in
          Don't have an account ? Create Account
          • Aspire Member Program
          • Connect: Lab, Data, Apps
          • Custom Products & Projects
          • Instrument Management
        • Home›
        • Golgi via cryo-tomography

        Inside the Golgi via cryo-tomography

        This video shows the 3D visualization of a Golgi apparatus from the green alga Chlamydomonas reinhardtii. The unicellular alga was flash-frozen without any artificial stains or fixatives. Prior to imaging with cryo-electron tomography (cryo-ET), a thin cryo-lamella sample was prepared from the vitrified cell by cryo-focused ion beam milling (cryo-FIB). The Golgi apparatus is an organelle located near the nucleus, serving as the major sorting center for the cell. It is made up of stacks of membranous sacs called cisternae, which are not connected to each other. The complex network of membranes and associated vesicles is known as the trans-Golgi network (TGN). Within the Golgi, proteins are modified, sorted and packaged; they undergo posttranslational modification through the process of glycosylation. The TGN consists of a cis face at which the proteins enter and a trans face from which the proteins exit. Upon exiting the Golgi apparatus, proteins are usually sent to either lysosomes, secretory granules or the plasma membrane, depending on their encoded signal. Vesicular transport within the Golgi complex is mediated by the coatomer protein I (COPI) complex. The COPI protein coat mediates trafficking of proteins from the cis end of the Golgi complex back to the rough endoplasmic reticulum and between Golgi cisternae.

        For the first time, cryo-ET has made it possible to determine the in situ structure of the COPI coat within vitrified Chlamydomonas reinhardtii cells. While the in situ structure supports the existing in vitro structural model (based on purified, reconstituted coatomer components), it also revealed bound cargo. Tomographic analysis of the native Golgi not only showed that the coat machinery remains constant during vesicle trafficking, but that vesicles change morphology and cargo content during their progression from the cis to the trans face. These findings were made possible by applying and combining several different methods within one workflow: cryo-FIB, cryo-ET and subtomogram averaging. Sample thinning by cryo-FIB is key for such an approach since it allows the production of sufficiently thin samples for cryo-ET in the first place. The Thermo Scientific Aquilos Cryo-FIB is a dedicated cryo-DualBeam microscope that facilitates the preparation of thin, electron-transparent lamellae from a target region within a vitrified cell.

        Data segmentation and visualization by Thermo Scientific Amira Software. Data courtesy of Dr. Benjamin Engel, Department of Molecular Structural Biology, Max Planck Institute for Biochemistry, Martinsried, Germany.

        Contact me with more information

        * Required field

        *
        *
        *
        *
         
         
        *
        *

         
         Speak with an account manager
         Request a demo
         Get a quote

         
         Email
         Phone

        To ensure that we are complying with your preferences, we need to confirm permission to send you communications by email.

         Please confirm you would like to receive marketing and promotional email messages about Thermo Fisher Scientific products and services.

        This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

         
         
        OrderingPlus Icon Minus Icon
        • Order Status
        • Order Help
        • Quick Order
        • Supply Center
        • eProcurement
        SupportPlus Icon Minus Icon
        • Help and Support
        • Contact Us
        • Technical Support Centers
        • Documents and Certificates
        • Report a Site Issue
        ResourcesPlus Icon Minus Icon
        • Learning Centers
        • Promotions
        • Events and Webinars
        • Social Media
        About Thermo FisherPlus Icon Minus Icon
        • About Us About Us
        • Careers Careers
        • Investors Investors
        • News News
        • Responsibility
        • Trademarks
        Our PortfolioPlus Icon Minus Icon
        • Thermo Scientific
        • Applied Biosystems
        • Invitrogen
        • Gibco
        • Ion Torrent
        • Fisher Scientific
        • Unity Lab Services
        • Patheon
        • PPD
        • Terms & Conditions
        • Privacy Information Center
        • Price & Freight Policy
        © Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved
        United States flag icon
        United States
        Sign in
        Don't have an account ? Create Account
        • Aspire Member Program
        • Connect: Lab, Data, Apps
        • Custom Products & Projects
        • Instrument Management