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        Liquid Extraction Surface Analysis (LESA) Mass Sectrometry of Intact Proteins

        In this webcast, Dr. Cooper discusses a novel workflow for spatial profiling of intact proteins on tissue slices utilizing native LESA MS to identify larger proteins (>47 kDa) than previous research, as well detecting and characterizing protein complexes with molecular weights ca. 103 kDa, demonstrating the softness of the ionization and transmission of protein complexes through FAIMS. Higher order MSn is performed to dissociate the complexes into subgroups, and sequence subgroups using multiple dissociation techniques. Combining FAIMS with LESA MS has enabled detection of hundreds of proteins in human liver sections.

        Key learning objectives

        • Improve data quality using differential ion mobility
        • Increase proteome profiling using FAIMS-LESA-Orbitrap workflow
        • Succeed at quantitative proteomic workflows for rapid top-down characterization

        Who should attend

        Researchers, Lab technicians and Directors including:

        • Director, PIs & Managers of mass spectrometry labs
        • Director, PIs & Managers using proteomics to investigate fundamental biology
        • Users interested in applying FAIMS to improve TMT, crosslinking, or proteomic workflows
        • Directors, PIs & Managers in Pharma/Biopharma evaluating drug-protein binding

        About the presenter

        Dr. Helen J. Cooper, Professor of Biosciences, University of Birmingham UK

        Dr. Helen J. Cooper, Professor of Biosciences, University of Birmingham UK

        Professor Helen J. Cooper is EPSRC Established Career Fellow and Professor of Mass Spectrometry at the University of Birmingham. She began her career at the University of Warwick where she obtained her PhD in Chemistry before undertaking postdoctoral research in Fourier transform mass spectrometry (FT-MS). She then moved to the National High Magnetic Field Laboratory at Florida State University where she continued her work in FT-MS. In 2003, she returned to the UK to establish an independent career at the University of Birmingham. Her research focus since has been the development and application of high-performance biological mass spectrometry. In 2004, she was awarded a Wellcome Trust University Technology Fellowship. At the end of her fellowship in 2010 she became Senior Lecturer. She became Reader in Mass Spectrometry in 2011 and Professor of Mass Spectrometry in 2013. In 2014, she was awarded her EPSRC Fellowship.

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        Resources

        • Comprehensive LESA Mass Spectrometry Imaging of Intact Proteins by Integration of Cylindrical FAIMS
        • LESA MS Imaging of Heat-Preserved and Frozen Tissue: Benefits of Multistep Static FAIMS
        • LESA FAIMS Mass Spectrometry for the Spatial Profiling of Proteins from Tissue
        • Accelerate your Proteomic Workflow with the Thermo Scientific FAIMS Pro Interface
        • eBook: Improving efficiency and sensitivity of mass spectrometry with FAIMS
        • Learn more about the Orbitrap Eclipse Tribrid mass spectrometer
        • Watch more LC-MS webinars
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