Liquid Extraction Surface Analysis (LESA) Mass Sectrometry of Intact Proteins

In this webcast, Dr. Cooper discusses a novel workflow for spatial profiling of intact proteins on tissue slices utilizing native LESA MS to identify larger proteins (>47 kDa) than previous research, as well detecting and characterizing protein complexes with molecular weights ca. 103 kDa, demonstrating the softness of the ionization and transmission of protein complexes through FAIMS. Higher order MSⁿ is performed to dissociate the complexes into subgroups, and sequence subgroups using multiple dissociation techniques. Combining FAIMS with LESA MS has enabled detection of hundreds of proteins in human liver sections.

Key learning objectives

Improve data quality using differential ion mobility
Increase proteome profiling using FAIMS-LESA-Orbitrap workflow
Succeed at quantitative proteomic workflows for rapid top-down characterization

Who should attend

Researchers, Lab technicians and Directors including:

Director, PIs & Managers of mass spectrometry labs
Director, PIs & Managers using proteomics to investigate fundamental biology
Users interested in applying FAIMS to improve TMT, crosslinking, or proteomic workflows
Directors, PIs & Managers in Pharma/Biopharma evaluating drug-protein binding

About the presenter

Dr. Helen J. Cooper, Professor of Biosciences, University of Birmingham UK

Professor Helen J. Cooper is EPSRC Established Career Fellow and Professor of Mass Spectrometry at the University of Birmingham. She began her career at the University of Warwick where she obtained her PhD in Chemistry before undertaking postdoctoral research in Fourier transform mass spectrometry (FT-MS). She then moved to the National High Magnetic Field Laboratory at Florida State University where she continued her work in FT-MS. In 2003, she returned to the UK to establish an independent career at the University of Birmingham. Her research focus since has been the development and application of high-performance biological mass spectrometry. In 2004, she was awarded a Wellcome Trust University Technology Fellowship. At the end of her fellowship in 2010 she became Senior Lecturer. She became Reader in Mass Spectrometry in 2011 and Professor of Mass Spectrometry in 2013. In 2014, she was awarded her EPSRC Fellowship.