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            Structural Tools In Your MS Tool Box

            Integrative methods in structural biology can generate molecular models for protein assemblies at any scale. The approach begins by levering existing structural knowledge as “building blocks”. It then incorporates different sources of biophysical data to fill in the gaps, armed with powerful computational routines.

            Mass spectrometry has enormous potential to serve as a key provider of structural restraints, and even push structural biology towards a cell-based activity. It can also provide critical conformational data that serves to link structure to function.

            We develop chemical labeling methods that anticipate structural biology at this scale, and we investigate ways in which the data can be used for assembling complex structures, in ways that can be delivered by specialized proteomics core labs.

            We’ll illustrate using examples drawn from our research in mitotic regulation, epigenetics and DNA damage repair mechanisms.

            During this webcast, the speaker will address:

            • Diversity of MS tools that are available for protein structural analysis
            • Use of integrated mass spectrometry techniques to enable characterization of complex and dynamic structure-function relationships.
            • Effective way of planning experiments and getting the most from current technology

            About the presenters

             David Schriemer, Professor, Dept. of Biochemistry & Molecular Biology University of Calgary

            David Schriemer, Professor, Dept. of Biochemistry & Molecular Biology University of Calgary

            Prof. Schriemer is a biochemist, with a PhD in Chemistry (1997). He spent several years in biotech before returning to academia, as a Canada Research Chair and is now building a new integrative structural biology facility for the study of DNA damage repair mechanisms.

             Dr. Jayshan Carpen, Moderator, Nature Research

            Dr. Jayshan Carpen, Moderator, Nature Research

            Jayshan received his Ph.D. in neurogenetics from the University of Surrey, UK. His doctoral thesis focused on identifying polymorphisms associated with diurnal preference and circadian sleep disorders. Jayshan worked as an events coordinator at the Royal Institution of Great Britain before moving to his current role in 2013 for Nature Publishing Group (NPG).

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            Resources

            • Protein Structure Analysis with Mass Spectrometry
            • Achieve even more with the Orbitrap Fusion Lumos Tribrid Mass Spectrometer
            • Integrative Structural Biology eBook
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