The data obtained in this study clearly show significant changes in metabolism arising from IPTG induction and subsequent protein production on the metabolic fingerprints of E. coli cells. However, it is important to note that due to the proof-of-principle nature of this research, it is difficult to determine if induction or protein production and subsequent extracellular transportation caused the associated metabolic changes observed. A larger secondary experiment is required to fully validate and confirm the biological findings.
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