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Advances in library preparation for Illumina NGS systems
Increasing statistical power in NGS studies using miniaturized library preparation
Dr. Stuart Levine, Director of the MIT BioMicro Center
The high cost of Illumina library production is a critical factor in encouraging the design of under-powered experiments. We have developed methodologies to dramatically lower library preparation cost that work for a broad number of library production techniques.
Assessing suitability of various DNA sources for monitoring leukemia activity with WGS
Dr. Karla Plevova, Department of Internal Medicine—Hematology and Oncology, University Hospital Brno; Central European Institute of Technology (CEITEC), Masaryk University
Using whole-genome next-generation sequencing we have investigated different sources of DNA obtained from chronic lymphocytic leukemia patients at various stages of their disease and related adverse conditions. Detection of personalized markers in cfDNA reflects neoplastic activity in tissue compartments other than peripheral blood and may indicate disease evolution requiring modification of treatment strategy. cfDNA provides the unique opportunity for disease monitoring which may direct further therapy decisions.
This project contains contributions from Tobias Rausch1, Marcela Zenatova2, Sarka Pospisilova2,3, Cristine Kinross4, Michael Doubek2,3, and Vladimir Benes1.
1. European Molecular Biology Laboratory (EMBL), Genomics Core Facility, Heidelberg, Germany
2. Department of Internal Medicine—Hematology and Oncology, University Hospital Brno, Brno, Czech Republic
3. Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czech Republic
4. Thermo Fisher Scientific Inc, California, United States