Cells and tissues that are cultured in a 3D environment showgreat potential to advance our understanding of complex biological processes. Organoids and spheroids are increasingly beingused in many research applications, including drug discovery, toxicology, disease modeling, and regenerative medicine. These models offer an opportunity to better understand complex biology in a physiologically relevant context where 2D models have not proven as successful.
This handbook provides an overview of the cell lines, culture conditions, and analysis techniques that are being used in the transition from 2D to 3D cell models. Whether you are just beginning this transition or are exploring improvements to existing 3D cell culture methods, we brought together a variety of resources to help you create better model systems for your experiments.
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