Specificity Comparison of AmpliTaq Gold® 360 DNA Polymerase to Polymerases From Other Suppliers

Summary of two replicates for each of 40 amplicons, indicating the average specificity for each amplicon and the standard deviation. AmpliTaq Gold® 360 PCR reactions were performed using 1 ng of template DNA per reaction and the manufacturer’s recommended cycling conditions.

Annealing and extension times and temperatures were specific to each primer set. Amplicons ranged from 300 to 1,400 bp in length, with an average length of 553 bp.

Data shown here compare AmpliTaq Gold® 360 DNA Polymerase (blue) with Roche FastStart® Taq DNA Polymerase (orange) and Sigma JumpStart™ Taq Polymerase (green).