Our four anti-prion monoclonal antibodies (6H4, 34C9, 2E11, and 15B3) are used in research laboratories worldwide, referenced in numerous scientific publications, and can be used with a wide variety of mammalian species such as cattle, sheep, rabbit, and primate. We also offer secondary antibodies (anti-swine, -bovine, and -chicken) as well as CSFV, BVDV (mAb 3 & 10), and FMDV (mAb 99, D5, A & Asia 1) antibodies.
Monoclonal antibody 15B3 (mAb 15B3)—mAb 15B3 can be used in immunoprecipitation experiments followed by detection using western blotting, FACS analysis, and real-time quaking-induced conversion (Orrú et al., 2011). Additionally, mAb 15B3 can be used as a capture antibody in sandwich ELISAs. The mouse monoclonal antibody 15B3 is a conformation-specific antibody that recognizes the disease-specific form of the prion protein (PrPSc) only (Korth et al., 1997; Nazor et al., 2005). The IgM isotype antibody can be used to detect PrPSc in brain homogenates without the need for Proteinase K digestion. This is of considerable importance for its application in blood, as it has been shown that prion infectivity in blood is sensitive to protease digestion and therefore might not be detected in methods using protease digestion. The group of Byron Caughey (Orrú et al., 2011) describes a very sensitive method for the detection of disease-specific prion proteins in blood or plasma. The enhanced quaking-induced conversion (eQuIC) assay uses amplification of prions together with a concentration step with the mAb 15B3 antibody.
Monoclonal antibody 6H4 (mAb 6H4)—mAb 6H4 is a proven, versatile anti-prion antibody that reacts with the prion protein of most mammalian species. mAb 6H4 recognizes the sequence DYEDRYYRE of the prion protein (human PrP: amino acids 144–152). This sequence is conserved in most known mammalian PrP sequences (human, cattle, sheep, rabbit, mink, and a variety of primates). In mouse, hamster, and rat, the tyrosine at position 145 is replaced by a tryptophan. However, mAb 6H4 reacts on western blots with mouse and hamster PrP, indicating that the substitution at position 145 does not prevent binding of 6H4. The antibody is cited in numerous scientific publications and applied in several methods, including western blot, ELISA, immunohistochemistry (IHC), immunoprecipitation, PET blot, and FACS analysis.
Antibodies—We provide a wide range of high-quality polyclonal and monoclonal antibodies against the classical swine fever virus (CSFV), as well as antibody conjugates. The antibodies are used for virus strain determination, staining of cryostat sections, and many other applications. Additionally, we offer four monoclonal antibodies for the detection of the foot and mouth disease virus, as well as two antibodies for the bovine viral diarrhea virus. These antibodies are suited for ELISA and western blot and are also available as POD conjugates. A wide range of monoclonal anti-swine, anti-bovine, and anti-chicken antibodies are also available for the detection of immunoglobulins.