Johne’s disease, or paratuberculosis, is a worldwide animal health problem affecting ruminants. It is caused by infection with Myobacterium avium spp. paratuberculosis (MAP). The presence of the disease can have serious production-limiting consequences and may cause significant economic loss in herds. The disease is difficult to diagnose because of long incubation times.
The identification of subclinical disease in animals, which can shed the organism over long periods and thus be the source of infection for other members of the herd, is crucial for disease control. The combined use of different diagnostic test methods, such as ELISA and real-time PCR tests, helps to identify and remove “shedders” earlier, thus helping to reduce the infection hazard for other healthy animals.
How has Mycobacterium avium ssp. paratuberculosis (MAP) been monitored in Saxony, Germany and how did real-time PCR perform in field testing? Presentation of Dr. Christina Boss, senior product manager of Dairy Solutions at Thermo Fisher Scientific, discusses the findings in the presentation of Dr. Andrea Konrath, Health and Veterinary Research Institute of Saxony, given at the Bacteriology AVID-Tagung in September 2015.
Dr. Esthel Frederic, consulting veterinarian of GDS18, France, talks about the development of an efficient MAP test protocol for accurate ruminant herd diagnosis. The team at GDS 18 conducted a study in which they compared results of diagnostic testing on individual goats to testing of environmental samples (pooled—bulk milk, milk filter, and swab socks sampled on the same herds).
Dr. Jean-Yves Houtain of ARSIA, Belgium, talks about a recent study using a highly sensitive detection method to assess the risk factors for MAP infections in very young calves. MAP infection in cattle occurs mainly during the first weeks of life. For efficient control of paratuberculosis in an infected herd, it is therefore important to prevent new infections among calves.
Dr. Petr Kralik of the Veterinary Research Institute, Czech Republic, explains how diagnostic tools can be used to control the disease through the early identification of paratuberculosis levels in animal herds. Paratuberculosis (Johne’s disease) can have a severe impact on the health of cattle and ruminant herds, which can contribute to significant economic losses.
The Applied Biosystems PrioCHECK MAP Ab 2.0 Plate Kit is the second generation of the first-approved Johne’s disease test that has been successfully used worldwide for over two decades. The test is used for both cattle and small ruminants. The PrioCHECK MAP Ab 2.0 Plate Kit is a high-throughput paratuberculosis test for cattle and small ruminants that combines speed and ease of use with a high diagnostic sensitivity and specificity.
The VetMAX real-time PCR tests are part of a fully integrated, single-vendor workflow that includes the Applied Biosystems MagMAX system for sample preparation and extraction and the Applied Biosystems 7500 Real-Time PCR System.
VetMAX MAP Screening Kit
The Applied Biosystems VetMAX MAP Screening Kit is a fast and accurate tool for the detection of MAP in feces samples of multiple species. The VetMAX MAP Screening Kit is registered by the Friedrich-Loeffler Institute (FLI).
VetMAX M.paratuberculosis 2.0 Kit
The Applied Biosystems VetMAX M.paratuberculosis 2.0 Kit is a screening real-time PCR test for the detection of MAP in multiple matrices and species.
VetMAX MAP IS900-F57 Kit
The Applied Biosystems VetMAX MAP IS900-F57 Kit offers rapid and specific detection of MAP and is used for epidemiological surveillance and the identification of shedding animals. In one single real-time PCR reaction you have two answers—screening and confirmation.
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