Achieve better separation efficiency under highly aqueous conditions
- Retention of hydrophilic compounds that are difficult to retain by Reversed Phase Liquid Chromatography (RPLC)
- Improved sensitivity with MS detection (due to more efficient desolvation of the analytes)
- Compatibility with SPE extracts which are often in 100% organic solvent
- Low back pressures due to the low viscosity of the organic-rich mobile phases
- Separation efficiency of very polar analytes in HILIC better than in RPLC under highly aqueous mobile phase
Comparison chromatograms
- Column: Thermo Scientific™ Hypersil GOLD™, 5µm, 150 x 2.1mm
- Mobile phase: H2O/ MeCN (98:2) + 0.1% formic acid
- Detection: +ESI (spray conditions adjusted for higher aqueous content of mobile phase)
- Injection: 1ng on column
- Column:Thermo Scientific™ Hypersil GOLD™ HILIC, 5µm, 150 x 2.1mm
- Mobile phase: Ammonium formate 50mM pH 3.5/ MeCN (10:90)
- Detection: +ESI Injection: 1ng on column
CMD SchemaApp code
