Charged Aerosol Detection for Liquid Chromatography

Sensitive, universal detection

Obtain sensitive, universal detection with a near-uniform response. Charged aerosol detectors measure a wide range of analytes in the areas of pharmaceuticals (large and small molecule), biomolecules, foods and beverages, specialty chemicals, and polymers. Their flexibility and performance are ideal for analytical R&D, while the detectors' simplicity and reproducibility benefit manufacturing QC/QA applications.

On this page

What makes charged aerosol detection a powerful choice?

What makes any detector useful is its ability to accurately measure a wide range of analytes. The problem with almost every other type of detector is that there is a bias. The bias might be that one analyte responds more than another or that mobile phase affects detection differently. A charged aerosol detector measures analyte charge that is in direct proportion to the amount of the analyte present.

Thermo Scientific™ charged aerosol detection technology can be used with the most up-to-date liquid chromatography instrumentation to measure analytes that cannot be seen by UV and may not be readily detected with other detection techniques, including molecules without chromophores. Features include:

  • Ability to detect non-volatile and many semi-volatile analytes
  • Consistent response independent of chemical structure
  • Applicability to both HPLC and UHPLC
  • Compatibility with gradient conditions
  • Sub-nanogram sensitivity
  • Simple and straightforward implementation in any lab setting
The problem: Detection gapsThe solution: Charged aerosol detection
No HPLC or UHPLC detection technique is perfect. UV detection is the most widely used, but it fails to detect compounds without chromophores. Other “universal detectors” do not combine application versatility with reliability. The result is detection gaps.Charged aerosol detection (CAD) technology delivers performance that refractive index (RI), low wavelength UV, and evaporative light scattering (ELS) detectors simply cannot match. A charged aerosol detector helps you see analytes that other technologies fail to detect.

This uncomplicated technique can both increase the efficiency of existing analytical operations and open up entirely new possibilities by exploiting a range of analytes unnoticed by other techniques.

How does charged aerosol detection work?

Charged Aerosol Detection measures charge not analytes

Featured CAD whiteboard videos

The detector converts the analyte molecules into dry particles. The number of particles increases proportionally with the amount of analyte.A stream of positively charged gas collides with the analyte particles. The charge is then transferred to the particles—the larger the particles, the greater the charge.The particles are transferred to a collector where the charge is measured by a highly-sensitive electrometer. This generates a signal in direct proportion to the quantity of analyte present.

What applications are ideal for charged aerosol detection?

A charged aerosol detector is the best choice for near universal detection for true quantitative analysis. This enables researchers to push the boundaries of their detection capabilities into areas where other techniques have trouble performing.

CAD technology is being used in nearly every industry where LC is utilized, from basic research to quality control. Because it offers more sensitivity, a wider dynamic range, and more consistent responses than other technologies, it can be used for many applications, including:

  • Biotech/Biopharmaceutical
  • Food/Beverage
  • Bioresearch
  • Natural products
  • Specialty chemicals
  • Pharmaceutical
  • Environmental
  • Biofuels

Whether you are measuring specific compound classes, such as proteins, lipids, carbohydrates, and small molecules, or involved in characterizing excipients and formulations, forced-degradation studies, testing for impurities, validating cleaning procedures, or evaluating final product quality—CAD technology is the right choice.

Charged aerosol detection bibliography

See how other labs use this novel detection method

Charged aerosol detection has the ability to measure any non-volatile and semi-volatile analytes at sub-nanogram levels, and does not require ionization or a chromophore. This bibliography highlights the breadth and scope of different analytical methods found in the literature.

Download bibliography

CMD SchemaApp code